• 1042 Citations
  • 13 h-Index
19982019

Research output per year

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Research Output

2019

Comparison between effects of retroactivity and resource competition upon change in downstream reporter genes of synthetic genetic circuits

Moriya, T., Yamaoka, T., Wakayama, Y., Ayukawa, S., Zhang, Z., Yamamura, M., Wakao, S. & Kiga, D., 2019 Mar 1, In : Life. 9, 1, 30.

Research output: Contribution to journalArticle

Open Access

Enhancement of binding affinity of folate to its receptor by peptide conjugation

Dharmatti, R., Miyatake, H., Nandakumar, A., Ueda, M., Kobayashi, K., Kiga, D., Yamamura, M. & Ito, Y., 2019 May 1, In : International journal of molecular sciences. 20, 9, 2152.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)

RNA world

Ayukawa, S., Enomoto, T. & Kiga, D., 2019 Jan 1, Astrobiology: From the Origins of Life to the Search for Extraterrestrial Intelligence. Springer Singapore, p. 77-90 14 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2018

A Highly Bioactive Lys-Deficient IFN Leads to a Site-Specific Di-PEGylated IFN with Equivalent Bioactivity to That of Unmodified IFN-α2b

Imada, T., Moriya, K., Uchiyama, M., Inukai, N., Hitotsuyanagi, M., Masuda, A., Suzuki, T., Ayukawa, S., Tagawa, Y. I., Dohmae, N., Kohara, M., Yamamura, M. & Kiga, D., 2018 Jan 1, (Accepted/In press) In : ACS Synthetic Biology.

Research output: Contribution to journalArticle

Escherichia coli expression, purification, and refolding of human folate receptor α (hFRα) and β (hFRβ)

Dharmatti, R., Miyatake, H., Zhang, C., Ren, X., Yumoto, A., Kiga, D., Yamamura, M. & Ito, Y., 2018 Sep 1, In : Protein Expression and Purification. 149, p. 17-22 6 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)

Horizontal transfer of code fragments between protocells can explain the origins of the genetic code without vertical descent

Froese, T., Campos, J. I., Fujishima, K., Kiga, D. & Virgo, N., 2018 Dec 1, In : Scientific Reports. 8, 1, 3532.

Research output: Contribution to journalArticle

2 Citations (Scopus)
2017
2 Citations (Scopus)
2016

A bacterial continuous culture system based on a microfluidic droplet open reactor

Ito, M., Sugiura, H., Ayukawa, S., Kiga, D. & Takinoue, M., 2016, In : Analytical Sciences. 32, 1, p. 61-66 6 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)

High-frequency noise attenuation of a two-component system responding to short-pulse input

Nishida, A., Sekine, R., Kiga, D. & Yamamura, M., 2016 Dec 19, Proceedings of the 2016 7th International Conference on Computational Systems-Biology and Bioinformatics, CSBio 2016. Association for Computing Machinery, Vol. Part F126331. p. 28-35 8 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Reproducibility of fluorescent expression from engineered biological constructs in E. coli

iGEM Interlab Study Contributors, 2016 Mar, In : PloS one. 11, 3, e0150182.

Research output: Contribution to journalArticle

18 Citations (Scopus)
2015

In vitro selection of a photoresponsive peptide aptamer to glutathione-immobilized microbeads

Tada, S., Zang, Q., Wang, W., Kawamoto, M., Liu, M., Iwashita, M., Uzawa, T., Kiga, D., Yamamura, M. & Ito, Y., 2015 Feb 1, In : Journal of Bioscience and Bioengineering. 119, 2, p. 137-139 3 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Two site genetic incorporation of varying length polyethylene glycol into the backbone of one peptide

Zang, Q., Tada, S., Uzawa, T., Kiga, D., Yamamura, M. & Ito, Y., 2015 Jul 31, In : Chemical Communications. 51, 76, p. 14385-14388 4 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)
2014

An observation method for autonomous signaling- mediated synthetic diversification in escherichia coli

Sekine, R., Ayukawa, S. & Kiga, D., 2014 Jan 1, In : Methods in Molecular Biology. 1151, p. 69-74 6 p.

Research output: Contribution to journalArticle

Construction and control of synthetic genetic circuits

Sekine, R. & Kiga, D., 2014 Jan 1, In : Seikagaku. 86, 2, p. 201-208 8 p.

Research output: Contribution to journalReview article

Effects of downstream genes on synthetic genetic circuits

Moriya, T., Yamamura, M. & Kiga, D., 2014 Dec 8, In : BMC Systems Biology. 8, 4, S4.

Research output: Contribution to journalArticle

4 Citations (Scopus)

Experimental Evolution of a Green Fluorescent Protein Composed of 19 Unique Amino Acids without Tryptophan

Kawahara-Kobayashi, A., Hitotsuyanagi, M., Amikura, K. & Kiga, D., 2014 Dec 11, In : Origins of Life and Evolution of Biospheres. 44, 2, p. 75-86 12 p.

Research output: Contribution to journalArticle

General Applicability of Synthetic Gene-Overexpression for Cell-Type Ratio Control via Reprogramming

Ishimatsu, K., Hata, T., Mochizuki, A., Sekine, R., Yamamura, M. & Kiga, D., 2014 Sep 19, In : ACS Synthetic Biology. 3, 9, p. 638-644 7 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

Multiple amino acid-excluded genetic codes for protein engineering using multiple sets of tRNA variants

Amikura, K., Sakai, Y., Asami, S. & Kiga, D., 2014 Mar 21, In : ACS Synthetic Biology. 3, 3, p. 140-144 5 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

Why 20 amino acids?

Amikura, K. & Kiga, D., 2014 Jun, In : Kobunshi. 63, 6, p. 392-393 2 p.

Research output: Contribution to journalArticle

2013

Cultivation of synthetic biology with the iGEM competition

Thamamongood, T., Lim, N. Z. L., Ho, T. Y. H., Ayukawa, S., Kiga, D. & Chow, K. L., 2013 Mar, In : Journal of Advanced Computational Intelligence and Intelligent Informatics. 17, 2, p. 161-166 6 p.

Research output: Contribution to journalReview article

Synthetic biology and dual use

Kiga, D., 2013, In : Journal of Disaster Research. 8, 4, p. 698-704 7 p.

Research output: Contribution to journalReview article

The number of amino acids in a genetic code

Amikura, K. & Kiga, D., 2013 Aug 21, In : RSC Advances. 3, 31, p. 12512-12517 6 p.

Research output: Contribution to journalReview article

2 Citations (Scopus)
2012

An aptazyme-based molecular device that converts a small-molecule input into an RNA output

Ayukawa, S., Sakai, Y. & Kiga, D., 2012 Jul 4, In : Chemical Communications. 48, 61, p. 7556-7558 3 p.

Research output: Contribution to journalArticle

13 Citations (Scopus)

Simplification of the genetic code: Restricted diversity of genetically encoded amino acids

Kawahara-Kobayashi, A., Masuda, A., Araiso, Y., Sakai, Y., Kohda, A., Uchiyama, M., Asami, S., Matsuda, T., Ishitani, R., Dohmae, N., Yokoyama, S., Kigawa, T., Nureki, O. & Kiga, D., 2012 Nov 1, In : Nucleic acids research. 40, 20, p. 10576-10584 9 p.

Research output: Contribution to journalArticle

14 Citations (Scopus)

Tunability of the ratio of cell states after the synthetic diversification by the diversity generator

Sekine, R., Yamamura, M., Hagiya, M. & Kiga, D., 2012 Jan 1, In : Communicative and Integrative Biology. 5, 4, p. 393-394 2 p.

Research output: Contribution to journalArticle

2011

RTRACS: A modularized RNA-dependent RNA transcription system with high programmability

Ayukawa, S., Takinoue, M. & Kiga, D., 2011 Dec 20, In : Accounts of Chemical Research. 44, 12, p. 1369-1379 11 p.

Research output: Contribution to journalArticle

19 Citations (Scopus)

Tunable synthetic phenotypic diversification on Waddington's landscape through autonomous signaling

Sekine, R., Yamamura, M., Ayukawa, S., Ishimatsu, K., Akama, S., Takinoue, M., Hagiya, M. & Kiga, D., 2011 Nov 1, In : Proceedings of the National Academy of Sciences of the United States of America. 108, 44, p. 17969-17973 5 p.

Research output: Contribution to journalArticle

23 Citations (Scopus)
2010

Construction of a genetic AND gate under a new standard for assembly of genetic parts

Ayukawa, S., Kobayashi, A., Nakashima, Y., Takagi, H., Hamada, S., Uchiyama, M., Yugi, K., Murata, S., Sakakibara, Y., Hagiya, M., Yamamura, M. & Kiga, D., 2010 Dec 2, In : BMC Genomics. 11, SUPPL. 4, S16.

Research output: Contribution to journalArticle

10 Citations (Scopus)
2009

Rna oscillator: Limit cycle oscillations based on artificial biomolecular reactions

Takinoue, M., Kiga, D., Shohda, K. I. & Suyama, A., 2009 Feb 1, In : New Generation Computing. 27, 2, p. 107-127 21 p.

Research output: Contribution to journalArticle

7 Citations (Scopus)
2008

A design and feasibility study of reactions comprising DNA molecular machine that walks autonomously by using a restriction enzyme

Sekiguchi, H., Komiya, K., Kiga, D. & Yamamura, M., 2008 Sep 1, In : Natural Computing. 7, 3, p. 303-315 13 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)

A realization of DNA molecular machine that walks autonomously by using a restriction enzyme

Sekiguchi, H., Komiya, K., Kiga, D. & Yamamura, M., 2008 Aug 27, DNA Computing - 13th International Meeting on DNA Computing, DNA13, Revised Selected Papers. p. 54-65 12 p. (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); vol. 4848 LNCS).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Experiments and simulation models of a basic computation element of an autonomous molecular computing system

Takinoue, M., Kiga, D., Shohda, K. I. & Suyama, A., 2008 Oct 29, In : Physical Review E - Statistical, Nonlinear, and Soft Matter Physics. 78, 4, 041921.

Research output: Contribution to journalArticle

35 Citations (Scopus)

Synthetic biology

Kiga, D. & Yamamura, M., 2008 Aug 1, In : New Generation Computing. 26, 4, p. 347-364 18 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)
2007

Oscillator for self-assembled DNA nanomachines

Takinoue, M., Kiga, D., Shohda, K. I. & Suyama, A., 2007 Dec 1, p. 104-106. 3 p.

Research output: Contribution to conferencePaper

SYANAC: SYnthetic biological Automaton for Noughts and Crosses

Ayukawa, S., Kobayashi, A., Nakashima, Y., Takagi, H., Hamada, S., Uchiyama, M., Yugi, K., Murata, S., Sakakibara, Y., Hagiya, M., Yamamura, M. & Kiga, D., 2007 Aug 15, In : IET Synthetic Biology. 1, 1-2, p. 64-67 4 p.

Research output: Contribution to journalArticle

2 Citations (Scopus)
2006

Cation-π interaction in the polyolefin cyclization cascade uncovered by incorporating unnatural amino acids into the catalytic sites of squalene cyclase

Morikubo, N., Fukuda, Y., Ohtake, K., Shinya, N., Kiga, D., Sakamoto, K., Asanuma, M., Hirota, H., Yokoyama, S. & Hoshino, T., 2006 Oct 11, In : Journal of the American Chemical Society. 128, 40, p. 13184-13194 11 p.

Research output: Contribution to journalArticle

57 Citations (Scopus)

DNA polymerase programmed with a hairpin DNA incorporates a multiple-instruction architecture into molecular computing

Komiya, K., Sakamoto, K., Kameda, A., Yamamoto, M., Ohuchi, A., Kiga, D., Yokoyama, S. & Hagiya, M., 2006 Jan 1, In : BioSystems. 83, 1, p. 18-25 8 p.

Research output: Contribution to journalArticle

19 Citations (Scopus)

Translation of 'rare' codons in a cell-free protein synthesis system from Escherichia coli

Chumpolkulwong, N., Sakamoto, K., Hayashi, A., Iraha, F., Shinya, N., Matsuda, N., Kiga, D., Urushibata, A., Shirouzu, M., Oki, K., Kigawa, T. & Yokoyama, S., 2006 Mar 1, In : Journal of structural and functional genomics. 7, 1, p. 31-36 6 p.

Research output: Contribution to journalArticle

22 Citations (Scopus)
2002

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

Kiga, D., Sakamoto, K., Kodama, K., Kigawa, T., Matsuda, T., Yabuki, T., Shirouzu, M., Harada, Y., Nakayama, H., Takio, K., Hasegawa, Y., Endo, Y., Hirao, I. & Yokoyama, S., 2002 Jul 23, In : Proceedings of the National Academy of Sciences of the United States of America. 99, 15, p. 9715-9720 6 p.

Research output: Contribution to journalArticle

137 Citations (Scopus)

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells

Sakamoto, K., Hayashi, A., Sakamoto, A., Kiga, D., Nakayama, H., Soma, A., Kobayashi, T., Kitabatake, M., Takio, K., Saito, K., Shirouzu, M., Hirao, I. & Yokoyama, S., 2002 Nov 1, In : Nucleic acids research. 30, 21, p. 4692-4699 8 p.

Research output: Contribution to journalArticle

173 Citations (Scopus)
2001

Shifted positioning of the anticodon nucleotide residues of amber suppressor tRNA species by Escherichia coli arginyl-tRNA synthetase

Kiga, D., Sakamoto, K., Sato, S., Hirao, I. & Yokoyama, S., 2001 Dec 22, In : European Journal of Biochemistry. 268, 23, p. 6207-6213 7 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)
2000

Molecular computation by DNA hairpin formation

Sakamoto, K., Gouzu, H., Komiya, K., Kiga, D., Yokoyama, S., Yokomori, T. & Hagiya, M., 2000 May 19, In : Science. 288, 5469, p. 1223-1226 4 p.

Research output: Contribution to journalArticle

320 Citations (Scopus)
1999

State transitions by molecules

Sakamoto, K., Kiga, D., Komiya, K., Gouzu, H., Yokoyama, S., Ikeda, S., Sugiyama, H. & Hagiya, M., 1999 Oct 1, In : BioSystems. 52, 1-3, p. 81-91 11 p.

Research output: Contribution to journalArticle

69 Citations (Scopus)
1998

An RNA aptamer to the xanthine/guanine base with a distinctive mode of purine recognition

Kiga, D., Futamura, Y., Sakamoto, K. & Yokoyama, S., 1998 Apr 1, In : Nucleic acids research. 26, 7, p. 1755-1760 6 p.

Research output: Contribution to journalArticle

61 Citations (Scopus)