Kuniki Kino

2019

Development of a synthesis method for odor sesquiterpenoid, (−)-rotundone, using non-heme Fe²⁺-chelate catalyst and ferric-chelate reductase

Umezawa, S., Konishi, S. & Kino, K., 2019 Jan 1, In : Bioscience, Biotechnology and Biochemistry. 83, 10, p. 1875-1883 9 p.

Research output: Contribution to journal › Article

Odors

Catalysts

Regeneration

Piper nigrum

Glucose 1-Dehydrogenase

2 Citations (Scopus)

Ectoine hydroxylase displays selective trans-3-hydroxylation activity towards l-proline

Hara, R., Nishikawa, T., Okuhara, T., Koketsu, K. & Kino, K., 2019 Jan 1, In : Applied Microbiology and Biotechnology.

Research output: Contribution to journal › Article

Hydroxylation

Mixed Function Oxygenases

Proline

Enzymes

Halomonas

Isolation and characterization of Gram-negative and Gram-positive bacteria capable of producing piceatannol from resveratrol

Furuya, T., Imaki, N., Shigei, K., Sai, M. & Kino, K., 2019 Jan 1, In : Applied Microbiology and Biotechnology.

Research output: Contribution to journal › Article

Gram-Positive Bacteria

Hydroxylation

Carbon

Growth

Arthrobacter

Synthesis of D-amino acid-containing dipeptides using the adenylation domains of nonribosomal peptide synthetase

Kano, S., Suzuki, S., Hara, R. & Kino, K., 2019 Jan 1, In : Applied and Environmental Microbiology. 85, 13, e00120-19.

Research output: Contribution to journal › Article

Peptide Synthases

dipeptides

Dipeptides

ligases

peptide

2018

6 Citations (Scopus)

A chemoenzymatic process for amide bond formation by an adenylating enzyme-mediated mechanism

Hara, R., Hirai, K., Suzuki, S. & Kino, K., 2018 Dec 1, In : Scientific Reports. 8, 1, 2950.

Research output: Contribution to journal › Article

Amides

Enzymes

Tryptophan

Amines

Food additives

4 Citations (Scopus)

Efficient monooxygenase-catalyzed piceatannol production: Application of cyclodextrins for reducing product inhibition

Furuya, T., Sai, M. & Kino, K., 2018 Jan 1, (Accepted/In press) In : Journal of Bioscience and Bioengineering.

Research output: Contribution to journal › Article

Cyclodextrins

Mixed Function Oxygenases

Resveratrol

Stilbenes

Hydroxylation

4 Citations (Scopus)

Production of aminoacyl prolines using the adenylation domain of nonribosomal peptide synthetase with class III polyphosphate kinase 2-mediated ATP regeneration

Suzuki, S., Hara, R. & Kino, K., 2018 Jun 1, In : Journal of Bioscience and Bioengineering. 125, 6, p. 644-648 5 p.

Research output: Contribution to journal › Article

Peptide Synthases

Adenosinetriphosphate

Proline

Peptides

Regeneration

Structural polymorphism of the Escherichia coli poly-α-L-glutamate synthetase RimK

Arimura, Y., Kono, T., Kino, K. & Kurumizaka, H., 2018 Jul 1, In : Acta Crystallographica Section F: Structural Biology Communications. 74, 7, p. 385-390 6 p.

Research output: Contribution to journal › Article

Glutamate Synthase (NADH)

glutamates

polymorphism

Escherichia

Polymorphism

2017

17 Citations (Scopus)

Biotechnological production of vanillin using immobilized enzymes

Furuya, T., Kuroiwa, M. & Kino, K., 2017 Feb 10, In : Journal of Biotechnology. 243, p. 25-28 4 p.

Research output: Contribution to journal › Article

Immobilized Enzymes

Enzymes

Coenzymes

ferulic acid

isoeugenol

2 Citations (Scopus)

Discovery of lysine hydroxylases in the clavaminic acid synthase-like superfamily for efficient hydroxylysine bioproduction

Hara, R., Yamagata, K., Miyake, R., Kawabata, H., Uehara, H. & Kino, K., 2017 Sep 1, In : Applied and Environmental Microbiology. 83, 17, e00693-17.

Research output: Contribution to journal › Article

2-Oxoglutarate 5-Dioxygenase Procollagen-Lysine

hydroxylysine

Hydroxylysine

industrial technology

Lysine

2016

7 Citations (Scopus)

Biocatalytic synthesis of 3,4,5,3',5'-pentahydroxy-trans-stilbene from piceatannol by two-component flavin-dependent monooxygenase HpaBC

Furuya, T., Sai, M. & Kino, K., 2016, In : Bioscience, Biotechnology and Biochemistry. 80, 1, p. 193-198 6 p.

Research output: Contribution to journal › Article

Stilbenes

Mixed Function Oxygenases

Escherichia coli

Mass Spectrometry

Buffers

5 Citations (Scopus)

Development of a multi-enzymatic cascade reaction for the synthesis of trans-3-hydroxy-l-proline from l-arginine

Hara, R., Kitatsuji, S., Yamagata, K. & Kino, K., 2016 Jan 1, In : Applied Microbiology and Biotechnology. 100, 1, p. 243-253 11 p.

Research output: Contribution to journal › Article

ornithine cyclodeaminase

Arginase

Proline

Arginine

Mixed Function Oxygenases

4 Citations (Scopus)

Effective production of Pro-Gly by mutagenesis of l-amino acid ligase

Kino, H., Nakajima, S., Arai, T. & Kino, K., 2016 Aug 1, In : Journal of Bioscience and Bioengineering. 122, 2, p. 155-159 5 p.

Research output: Contribution to journal › Article

prolylglycine

Mutagenesis

Ligases

Amino acids

Amino Acids

3 Citations (Scopus)

Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase

Watanabe, S., Tajima, K., Fujii, S., Fukumori, F., Hara, R., Fukuda, R., Miyazaki, M., Kino, K. & Watanabe, Y., 2016 Dec 8, In : Scientific Reports. 6, 38720.

Research output: Contribution to journal › Article

Aconitate Hydratase

Hydro-Lyases

Proline

Hydroxyproline

Cysteine

2015

6 Citations (Scopus)

Alteration of the substrate specificity of cytochrome P450 CYP199A2 by site-directed mutagenesis

Furuya, T., Shitashima, Y. & Kino, K., 2015 Jan 1, In : Journal of Bioscience and Bioengineering. 119, 1, p. 47-51 5 p.

Research output: Contribution to journal › Article

Mutagenesis

Substrate Specificity

Site-Directed Mutagenesis

Cytochrome P-450 Enzyme System

Carboxylic acids

6 Citations (Scopus)

Alteration of the substrate specificity of L-amino acid ligase and selective synthesis of Met-Gly as a salt taste enhancer

Kino, H. & Kino, K., 2015, In : Bioscience, Biotechnology and Biochemistry. 79, 11, p. 1827-1832 6 p.

Research output: Contribution to journal › Article

methionylglycine

methionylmethionine

Ligases

Substrate Specificity

Salts

3 Citations (Scopus)

Catalytic function of the mycobacterial binuclear iron monooxygenase in acetone metabolism

Furuya, T., Nakao, T. & Kino, K., 2015 Aug 31, In : FEMS Microbiology Letters. 362, 19, fnv136.

Research output: Contribution to journal › Article

Acetone

Iron

Mycobacterium

Escherichia coli

Mycobacterium smegmatis

6 Citations (Scopus)

Enzymatic carboxylation of hydroxystilbenes by the γ-resorcylic acid decarboxylase from Rhizobium radiobacter WU-0108 under reverse reaction conditions

Sato, M., Sakurai, N., Suzuki, H., Shibata, D. & Kino, K., 2015 Dec 1, In : Journal of Molecular Catalysis B: Enzymatic. 122, p. 348-352 5 p.

Research output: Contribution to journal › Article

Resveratrol

Carboxylation

Agrobacterium tumefaciens

Carboxy-Lyases

Benzoic acid

19 Citations (Scopus)

High-yield production of vanillin from ferulic acid by a coenzyme-independent decarboxylase/oxygenase two-stage process

Furuya, T., Miura, M., Kuroiwa, M. & Kino, K., 2015 May 5, In : New Biotechnology. 32, 3, p. 335-339 5 p.

Research output: Contribution to journal › Article

ferulic acid

Coenzymes

Oxygenases

Carboxy-Lyases

Escherichia coli

8 Citations (Scopus)

Hydroxamate-based colorimetric assay to assess amide bond formation by adenylation domain of nonribosomal peptide synthetases

Hara, R., Suzuki, R. & Kino, K., 2015 May 15, In : Analytical Biochemistry. 477, p. 89-91 3 p.

Research output: Contribution to journal › Article

Peptide Synthases

Biosynthesis

Amides

Tyrocidine

Assays

3 Citations (Scopus)

One-pot production of L-threo-3-hydroxyaspartic acid using asparaginase-deficient Escherichia coli expressing asparagine hydroxylase of Streptomyces coelicolor A3(2)

Hara, R., Nakano, M. & Kino, K., 2015, In : Applied and Environmental Microbiology. 81, 11, p. 3648-3654 7 p.

Research output: Contribution to journal › Article

Streptomyces coelicolor

asparaginase

Asparaginase

Asparagine

asparagine

14 Citations (Scopus)

Refined regio- and stereoselective hydroxylation of l -pipecolic acid by protein engineering of l -proline cis -4-hydroxylase based on the X-ray crystal structure

Koketsu, K., Shomura, Y., Moriwaki, K., Hayashi, M., Mitsuhashi, S., Hara, R., Kino, K. & Higuchi, Y., 2015 Apr 17, In : ACS Synthetic Biology. 4, 4, p. 383-392 10 p.

Research output: Contribution to journal › Article

Protein Engineering

Hydroxylation

Mixed Function Oxygenases

Proline

Hydroxyproline

2 Citations (Scopus)

Screening of salt taste enhancing dipeptides based on a new strategy using L-amino acid ligase

Kino, H., Kakutani, M., Hattori, K., Tojo, H., Komai, T., Nammoku, T. & Kino, K., 2015 May 1, In : Nippon Shokuhin Kagaku Kogaku Kaishi. 62, 6, p. 274-281 8 p.

Research output: Contribution to journal › Article

dipeptides

Dipeptides

Ligases

ligases

Salts

4 Citations (Scopus)

Structure of RizA, an l-amino-acid ligase from Bacillus subtilis

Kagawa, W., Arai, T., Ishikura, S., Kino, K. & Kurumizaka, H., 2015 Sep 1, In : Acta Crystallographica Section F:Structural Biology Communications. 71, p. 1125-1130 6 p.

Research output: Contribution to journal › Article

Bacillus

Bacilli

Ligases

Bacillus subtilis

amino acids

2014

18 Citations (Scopus)

A coenzyme-independent decarboxylase/oxygenase cascade for the efficient synthesis of vanillin

Furuya, T., Miura, M. & Kino, K., 2014 Oct 13, In : Chembiochem : a European journal of chemical biology. 15, 15, p. 2248-2254 7 p.

Research output: Contribution to journal › Article

ferulic acid

Oxygenases

Carboxy-Lyases

Coenzymes

Flavor compounds

28 Citations (Scopus)

Catalytic activity of the two-component flavin-dependent monooxygenase from Pseudomonas aeruginosa toward cinnamic acid derivatives

Furuya, T. & Kino, K., 2014, In : Applied Microbiology and Biotechnology. 98, 3, p. 1145-1154 10 p.

Research output: Contribution to journal › Article

Mixed Function Oxygenases

Coumaric Acids

Pseudomonas aeruginosa

ferulic acid

Escherichia coli

7 Citations (Scopus)

Identification and characterization of 2-oxoglutarate-dependent dioxygenases catalyzing selective cis-hydroxylation of proline and pipecolinic acid from actinomycetes

Hara, R., Uchiumi, N. & Kino, K., 2014 Feb 20, In : Journal of Biotechnology. 172, 1, p. 55-58 4 p.

Research output: Contribution to journal › Article

Dioxygenases

Hydroxylation

Actinobacteria

Proline

Hydroxyproline

7 Citations (Scopus)

Regio- and stereoselective oxygenation of proline derivatives by using microbial 2-oxoglutarate-dependent dioxygenases

Hara, R., Uchiumi, N., Okamoto, N. & Kino, K., 2014, In : Bioscience, Biotechnology, and Biochemistry. 78, 8, p. 1384-1388 5 p.

Research output: Contribution to journal › Article

Dioxygenases

Oxygenation

Proline

Derivatives

Azetidinecarboxylic Acid

19 Citations (Scopus)

Regioselective synthesis of piceatannol from resveratrol: Catalysis by two-component flavin-dependent monooxygenase HpaBC in whole cells

Furuya, T. & Kino, K., 2014 Apr 23, In : Tetrahedron Letters. 55, 17, p. 2853-2855 3 p.

Research output: Contribution to journal › Article

Mixed Function Oxygenases

Catalysis

Stilbenes

Hydroxylation

Derivatives

2013

5 Citations (Scopus)

Application of protein N-terminal amidase in enzymatic synthesis of dipeptides containing acidic amino acids specifically at the N-terminus

Arai, T., Noguchi, A., Takano, E. & Kino, K., 2013 Apr, In : Journal of Bioscience and Bioengineering. 115, 4, p. 382-387 6 p.

Research output: Contribution to journal › Article

amidase

Acidic Amino Acids

Dipeptides

Amino acids

aspartyl-phenylalanine

1 Citation (Scopus)

Biocatalytic production of 5-hydroxy-2-adamantanone by P450cam coupled with NADH regeneration

Furuya, T., Kanno, T., Yamamoto, H., Kimoto, N., Matsuyama, A. & Kino, K., 2013, In : Journal of Molecular Catalysis B: Enzymatic. 94, p. 111-118 8 p.

Research output: Contribution to journal › Article

Camphor 5-Monooxygenase

NAD

Glucose

Regeneration

Glucose 1-Dehydrogenase

12 Citations (Scopus)

Construction of photoenergetic mitochondria in cultured mammalian cells.

Hara, K. Y., Wada, T., Kino, K., Asahi, T. & Sawamura, N., 2013, In : Scientific Reports. 3, p. 1635 1 p.

Research output: Contribution to journal › Article

Proton-Motive Force

Cultured Cells

Mitochondria

Rhodopsin

Proton Pumps

11 Citations (Scopus)

Enhanced synthesis of 5-Hydroxy-L-tryptophan through tetrahydropterin regeneration

Hara, R. & Kino, K., 2013, In : AMB Express. 3, p. 1-7 7 p.

Research output: Contribution to journal › Article

5-Hydroxytryptophan

Tryptophan

Regeneration

Pterins

Tryptophanase

Enzymatic production of designed peptide

Kino, K., 2013 Nov 1, Microbial Production: From Genome Design to Cell Engineering. Springer Japan, Vol. 9784431546078. p. 191-204 14 p.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Oligopeptides

Dipeptides

Peptides

aspartyl-phenylalanine

Amino Acids

18 Citations (Scopus)

L-Amino acid ligase from Pseudomonas syringae producing tabtoxin can be used for enzymatic synthesis of various functional peptides

Arai, T., Arimura, Y., Ishikura, S. & Kino, K., 2013 Aug, In : Applied and Environmental Microbiology. 79, 16, p. 5023-5029 7 p.

Research output: Contribution to journal › Article

Pseudomonas syringae

Ligases

ligases

peptide

amino acid

9 Citations (Scopus)

Microbial production of N-acetyl cis-4-hydroxy-l-proline by coexpression of the Rhizobium l-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1

Bach, T. M. H., Hara, R., Kino, K., Ohtsu, I., Yoshida, N. & Takagi, H., 2013 Jan, In : Applied Microbiology and Biotechnology. 97, 1, p. 247-257 11 p.

Research output: Contribution to journal › Article

Rhizobium

Acetyltransferases

Mixed Function Oxygenases

Proline

Yeasts

10 Citations (Scopus)

NRPSs and amide ligases producing homopoly(amino acid)s and homooligo(amino acid)s

Hamano, Y., Arai, T., Ashiuchi, M. & Kino, K., 2013, In : Natural Product Reports. 30, 8, p. 1087-1097 11 p.

Research output: Contribution to journal › Article

Ligases

Amides

Amino Acids

Prostaglandins A

Lysine

5 Citations (Scopus)

Reconstitution of active mycobacterial binuclear iron monooxygenase complex in escherichia coli

Furuya, T., Hayashi, M. & Kino, K., 2013, In : Applied and Environmental Microbiology. 79, 19, p. 6033-6039 7 p.

Research output: Contribution to journal › Article

Mixed Function Oxygenases

Iron

iron

Escherichia coli

oxidation

9 Citations (Scopus)

The mycobacterial binuclear iron monooxygenases require a specific chaperonin-like protein for functional expression in a heterologous host

Furuya, T., Hayashi, M., Semba, H. & Kino, K., 2013 Feb, In : FEBS Journal. 280, 3, p. 817-826 10 p.

Research output: Contribution to journal › Article

Chaperonins

Acinetobacter

Mixed Function Oxygenases

Iron

Genes

2012

28 Citations (Scopus)

Biotechnological production of caffeic acid by bacterial cytochrome P450 CYP199A2

Furuya, T., Arai, Y. & Kino, K., 2012 Sep, In : Applied and Environmental Microbiology. 78, 17, p. 6087-6094 8 p.

Research output: Contribution to journal › Article

caffeic acid

cytochrome P-450

Cytochrome P-450 Enzyme System

cytochrome

mutants

28 Citations (Scopus)

Novel CCK-dependent vasorelaxing dipeptide, Arg-Phe, decreases blood pressure and food intake in rodents

Kagebayashi, T., Kontani, N., Yamada, Y., Mizushige, T., Arai, T., Kino, K. & Ohinata, K., 2012 Sep, In : Molecular Nutrition and Food Research. 56, 9, p. 1456-1463 8 p.

Research output: Contribution to journal › Article

cholecystokinin

dipeptides

Dipeptides

Cholecystokinin

blood pressure

14 Citations (Scopus)

Purification, characterization, and gene identification of an α-glucosyl transfer enzyme, a novel type α-glucosidase from Xanthomonas campestris WU-9701

Sato, T., Hasegawa, N., Saito, J., Umezawa, S., Honda, Y., Kino, K. & Kirimura, K., 2012 Aug, In : Journal of Molecular Catalysis B: Enzymatic. 80, p. 20-27 8 p.

Research output: Contribution to journal › Article

Xanthomonas campestris

Glucosidases

Maltose

Purification

Enzymes

8 Citations (Scopus)

The structure of l-amino-acid ligase from Bacillus licheniformis

Suzuki, M., Takahashi, Y., Noguchi, A., Arai, T., Yagasaki, M., Kino, K. & Saito, J. I., 2012 Nov, In : Acta Crystallographica Section D: Biological Crystallography. 68, 11, p. 1535-1540 6 p.

Research output: Contribution to journal › Article

Ligases

Dipeptides

Amino Acids

Substrate Specificity

Bacillus licheniformis

2011

3 Citations (Scopus)

ATP Photosynthetic vesicles for light-driven bioprocesses

Hara, K. Y., Suzuki, R., Suzuki, T., Yoshida, M. & Kino, K., 2011 Jun, In : Biotechnology Letters. 33, 6, p. 1133-1138 6 p.

Research output: Contribution to journal › Article

Adenosinetriphosphate

Adenosine Triphosphate

Light

Hexokinase

Rhodopsin

20 Citations (Scopus)

Identification of the monooxygenase gene clusters responsible for the regioselective oxidation of phenol to hydroquinone in mycobacteria

Furuya, T., Hirose, S., Osanai, H., Semba, H. & Kino, K., 2011 Feb, In : Applied and Environmental Microbiology. 77, 4, p. 1214-1220 7 p.

Research output: Contribution to journal › Article

hydroquinone

Mycobacterium

Mycobacterium smegmatis

Multigene Family

Mixed Function Oxygenases

4 Citations (Scopus)

Identification of the regulator gene responsible for the acetone-responsive expression of the binuclear iron monooxygenase gene cluster in mycobacteria

Furuya, T., Hirose, S., Semba, H. & Kino, K., 2011 Oct, In : Journal of Bacteriology. 193, 20, p. 5817-5823 7 p.

Research output: Contribution to journal › Article

Mycobacterium smegmatis

Regulator Genes

Mycobacterium

Multigene Family

Acetone

14 Citations (Scopus)

Poly-α-glutamic acid synthesis using a novel catalytic activity of Rimk from Escherichia coli K-12

Kino, K., Arai, T. & Arimura, Y., 2011 Mar, In : Applied and Environmental Microbiology. 77, 6, p. 2019-2025 7 p.

Research output: Contribution to journal › Article

polyglutamic acid

Escherichia coli K12

catalytic activity

Glutamic Acid

amino acid

Structure-based modification of D-alanine-D-alanine ligase from Thermotoga maritima ATCC 43589 for depsipeptide synthesis

Nakagawa, T., Satake, R., Sato, M. & Kino, K., 2011, In : Bioscience, Biotechnology and Biochemistry. 75, 4, p. 700-704 5 p.

Research output: Contribution to journal › Article

Thermotoga maritima

Depsipeptides

Mutagenesis

Polymers

Substitution reactions

2010

14 Citations (Scopus)

A novel L-amino acid ligase from bacillus subtilis NBRC3134 catalyzed oligopeptide synthesis

Kino, K., Arai, T. & Tateiwa, D., 2010, In : Bioscience, Biotechnology and Biochemistry. 74, 1, p. 129-134 6 p.

Research output: Contribution to journal › Article

Oligopeptides

Bacilli

Ligases

Bacillus subtilis

Amino Acids

29 Citations (Scopus)

Genome mining approach for the discovery of novel cytochrome P450 biocatalysts

Furuya, T. & Kino, K., 2010 Apr, In : Applied Microbiology and Biotechnology. 86, 4, p. 991-1002 12 p.

Research output: Contribution to journal › Article

Cytochrome P-450 Enzyme System

Genome

Enzymes

Microbial Genome

Oxygen