βν integrin inhibits chronic and high level activation of JNK to repress senescence phenotypes in Drosophila adult midgut

Takashi Okumura, Koji Takeda, Kiichiro Taniguchi, Takashi Adachi-Yamada

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Proper control of adult stem cells including their proliferation and differentiation is crucial in maintaining homeostasis of well-organized tissues/organs throughout an organism's life. The Drosophila adult midgut has intestinal stem cells (ISCs), which have been exploited as a simple model system to investigate mechanisms controlling adult tissue homeostasis. Here, we found that a viable mutant of βν integrin (βint-ν), encoding one of two Drosophila integrin β subunits, showed a short midgut and abnormal multilayered epithelia accompanied by an increase in ISC proliferation and misdifferentiation defects. The increase in ISC proliferation and misdifferentiation was due to frequent ISC duplication expanding a pool of ISCs, which was caused by depression of the Notch signalling, and up-regulation of unpaired (upd), a gene encoding an extracellular ligand in the JAK/STAT signalling pathway. In addition, we observed that abnormally high accumulation of filamentous actin (F-actin) was caused in the βint-ν mutant enterocytes. Furthermore, the defects were rescued by suppressing c-Jun N-terminal kinase (JNK) signalling, which was up-regulated in a manner correlated with the defect levels in the above-mentioned βint-ν mutant phenotype. These symptoms observed in young βint-ν mutant midgut were very similar to those in the aged midgut in wild type. Our results suggested that βint-ν has a novel function for the Drosophila adult midgut homeostasis under normal conditions and provided a new insight into possible age-related diseases caused by latent abnormality of an integrin function.

Original languageEnglish
Article numbere89387
JournalPLoS One
Volume9
Issue number2
DOIs
Publication statusPublished - 2014 Feb 20
Externally publishedYes

Fingerprint

integrins
Integrins
midgut
Drosophila
Stem cells
phosphotransferases (kinases)
Phosphotransferases
Chemical activation
Phenotype
phenotype
stem cells
Stem Cells
homeostasis
mutants
Homeostasis
Cell proliferation
Defects
Tissue homeostasis
cell proliferation
Cell Proliferation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

βν integrin inhibits chronic and high level activation of JNK to repress senescence phenotypes in Drosophila adult midgut. / Okumura, Takashi; Takeda, Koji; Taniguchi, Kiichiro; Adachi-Yamada, Takashi.

In: PLoS One, Vol. 9, No. 2, e89387, 20.02.2014.

Research output: Contribution to journalArticle

Okumura, Takashi ; Takeda, Koji ; Taniguchi, Kiichiro ; Adachi-Yamada, Takashi. / βν integrin inhibits chronic and high level activation of JNK to repress senescence phenotypes in Drosophila adult midgut. In: PLoS One. 2014 ; Vol. 9, No. 2.
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