β-actinin isoforms in various types of muscle and non-muscle tissues

Yoko Asami, Takashi Funatsu, Shin'ichi Ishiwata

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    We found that β-actinin isoforms are present in various types of tissues in adult chicken by using immunoblotting after two dimensional gel electrophoresis; for this purpose, an antibody was raised against β-actinin purified from adult chicken breast muscle (pectoralis major). One of the β-actinin subunits, βI, was present in all tissues we examined, i.e. skeletal (pectoralis major, semitendinosus, and anterior latissimus dorsi), cardiac, and smooth (gizzard) muscles, non-muscle (brain, liver, and kidney) tissues and blood, whereas another subunit, β11, was present only in muscle tissues. A new subunit (designated βIII) that was found in the embryonic stages of skeletal muscle (Asami, Funatsu & Ishiwata (1988) J. Biochem. 103, 72-75) was present instead of βII in non-muscle tissues and blood. In cardiac and smooth muscles, βIII coexisted with βI and βII. The antibody of β-actinin did not cross-react to cytoplasmic β-actinin (molecular weight, 80, 000 daltons) found in kidney. It was suggested that the combination of βI and βIII present in non-muscle tissues and blood is identical to the barbed end capping protein isolated from brain by Killiman and Isenberg (EMBO J. 1,889-894 (1982)). It is likely that β-actinin forms a genetic family whose constituents have an ability to cap either the pointed or barbed end of actin filaments.

    Original languageEnglish
    Pages (from-to)76-80
    Number of pages5
    JournalJournal of Biochemistry
    Volume103
    Issue number1
    Publication statusPublished - 1988 Jan

    Fingerprint

    Actinin
    Muscle
    Smooth muscle
    Blood
    Protein Isoforms
    Kidney
    Tissue
    Antibody
    Muscles
    Cardiac muscle
    Skeletal muscle
    Actin
    Filament
    Electrophoresis
    Antibodies
    Cardiac
    Liver
    Smooth Muscle
    Chickens
    Brain

    ASJC Scopus subject areas

    • Statistics, Probability and Uncertainty
    • Applied Mathematics
    • Physiology (medical)
    • Radiology Nuclear Medicine and imaging
    • Molecular Biology
    • Biochemistry

    Cite this

    Asami, Y., Funatsu, T., & Ishiwata, S. (1988). β-actinin isoforms in various types of muscle and non-muscle tissues. Journal of Biochemistry, 103(1), 76-80.

    β-actinin isoforms in various types of muscle and non-muscle tissues. / Asami, Yoko; Funatsu, Takashi; Ishiwata, Shin'ichi.

    In: Journal of Biochemistry, Vol. 103, No. 1, 01.1988, p. 76-80.

    Research output: Contribution to journalArticle

    Asami, Y, Funatsu, T & Ishiwata, S 1988, 'β-actinin isoforms in various types of muscle and non-muscle tissues', Journal of Biochemistry, vol. 103, no. 1, pp. 76-80.
    Asami, Yoko ; Funatsu, Takashi ; Ishiwata, Shin'ichi. / β-actinin isoforms in various types of muscle and non-muscle tissues. In: Journal of Biochemistry. 1988 ; Vol. 103, No. 1. pp. 76-80.
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