β-carotene production in a novel hydrogen-producing marine photosynthetic bacterium Rhodovulum sulfidophilum expressing the Erythrobacter longus OCh101 crt I and crt Y genes

Haruko Takeyama, Jeanne Sunarjo, Akiyo Yamada, Haruo Matsumura, Etsuko Kusakabe, Tadashi Matsunaga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Gene transfer in a novel hydrogen-producing marine photosynthetic bacterium, Rhodovulum sulfidophilum, was investigated using the conjugal vector pSUP1021 (Tn5::Km). Kanamycin-resistance transconjugants were obtained at an efficiency of 2.4 × 10-4 at a mating ratio of 1:10 (E. coli-Rv. sulfidophilum), and the insertion of Tn5 into the chromosomal DNA was confirmed by Southern hybridization. To further establish the gene transfer system in this strain, cloning and expression of the Erythrobacter longus OCh101 crt I and crt Y genes were carried out. The plasmid pRKY415, which carries the E. longus OCh101 crt I and crt Y genes, was stably maintained in Rv. sulfidophilum without significant structural modifications, and the expression of the crt I and crt Y genes altered the original carotenoid biosynthetic pathway of this strain, resulting in the production of β-carotene (20 μg/g dry cells).

Original languageEnglish
Pages (from-to)224-229
Number of pages6
JournalJournal of Marine Biotechnology
Volume4
Issue number4
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Rhodovulum
Sphingomonadaceae
Carotenoids
Gene transfer
Hydrogen
Bacteria
Genes
Kanamycin
Cloning
Escherichia coli
Kanamycin Resistance
Plasmids
Biosynthetic Pathways
DNA
Organism Cloning

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

β-carotene production in a novel hydrogen-producing marine photosynthetic bacterium Rhodovulum sulfidophilum expressing the Erythrobacter longus OCh101 crt I and crt Y genes. / Takeyama, Haruko; Sunarjo, Jeanne; Yamada, Akiyo; Matsumura, Haruo; Kusakabe, Etsuko; Matsunaga, Tadashi.

In: Journal of Marine Biotechnology, Vol. 4, No. 4, 1996, p. 224-229.

Research output: Contribution to journalArticle

@article{cf5823239c9c423dbd9b2da7069bb70f,
title = "β-carotene production in a novel hydrogen-producing marine photosynthetic bacterium Rhodovulum sulfidophilum expressing the Erythrobacter longus OCh101 crt I and crt Y genes",
abstract = "Gene transfer in a novel hydrogen-producing marine photosynthetic bacterium, Rhodovulum sulfidophilum, was investigated using the conjugal vector pSUP1021 (Tn5::Km). Kanamycin-resistance transconjugants were obtained at an efficiency of 2.4 × 10-4 at a mating ratio of 1:10 (E. coli-Rv. sulfidophilum), and the insertion of Tn5 into the chromosomal DNA was confirmed by Southern hybridization. To further establish the gene transfer system in this strain, cloning and expression of the Erythrobacter longus OCh101 crt I and crt Y genes were carried out. The plasmid pRKY415, which carries the E. longus OCh101 crt I and crt Y genes, was stably maintained in Rv. sulfidophilum without significant structural modifications, and the expression of the crt I and crt Y genes altered the original carotenoid biosynthetic pathway of this strain, resulting in the production of β-carotene (20 μg/g dry cells).",
author = "Haruko Takeyama and Jeanne Sunarjo and Akiyo Yamada and Haruo Matsumura and Etsuko Kusakabe and Tadashi Matsunaga",
year = "1996",
language = "English",
volume = "4",
pages = "224--229",
journal = "Journal of Marine Biotechnology",
issn = "0941-2905",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - β-carotene production in a novel hydrogen-producing marine photosynthetic bacterium Rhodovulum sulfidophilum expressing the Erythrobacter longus OCh101 crt I and crt Y genes

AU - Takeyama, Haruko

AU - Sunarjo, Jeanne

AU - Yamada, Akiyo

AU - Matsumura, Haruo

AU - Kusakabe, Etsuko

AU - Matsunaga, Tadashi

PY - 1996

Y1 - 1996

N2 - Gene transfer in a novel hydrogen-producing marine photosynthetic bacterium, Rhodovulum sulfidophilum, was investigated using the conjugal vector pSUP1021 (Tn5::Km). Kanamycin-resistance transconjugants were obtained at an efficiency of 2.4 × 10-4 at a mating ratio of 1:10 (E. coli-Rv. sulfidophilum), and the insertion of Tn5 into the chromosomal DNA was confirmed by Southern hybridization. To further establish the gene transfer system in this strain, cloning and expression of the Erythrobacter longus OCh101 crt I and crt Y genes were carried out. The plasmid pRKY415, which carries the E. longus OCh101 crt I and crt Y genes, was stably maintained in Rv. sulfidophilum without significant structural modifications, and the expression of the crt I and crt Y genes altered the original carotenoid biosynthetic pathway of this strain, resulting in the production of β-carotene (20 μg/g dry cells).

AB - Gene transfer in a novel hydrogen-producing marine photosynthetic bacterium, Rhodovulum sulfidophilum, was investigated using the conjugal vector pSUP1021 (Tn5::Km). Kanamycin-resistance transconjugants were obtained at an efficiency of 2.4 × 10-4 at a mating ratio of 1:10 (E. coli-Rv. sulfidophilum), and the insertion of Tn5 into the chromosomal DNA was confirmed by Southern hybridization. To further establish the gene transfer system in this strain, cloning and expression of the Erythrobacter longus OCh101 crt I and crt Y genes were carried out. The plasmid pRKY415, which carries the E. longus OCh101 crt I and crt Y genes, was stably maintained in Rv. sulfidophilum without significant structural modifications, and the expression of the crt I and crt Y genes altered the original carotenoid biosynthetic pathway of this strain, resulting in the production of β-carotene (20 μg/g dry cells).

UR - http://www.scopus.com/inward/record.url?scp=8744283743&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8744283743&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:8744283743

VL - 4

SP - 224

EP - 229

JO - Journal of Marine Biotechnology

JF - Journal of Marine Biotechnology

SN - 0941-2905

IS - 4

ER -