βIV-spectrin forms a diffusion barrier against L1CAM at the axon initial segment

Kazunari Nishimura, Hiroki Akiyama, Masayuki Komada, Hiroyuki Kamiguchi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Axonal and somatodendritic plasma membranes of polarized neurons express distinct sets of functional molecules. It is known that the neuronal polarity can be maintained by a barrier that impedes diffusional mixing of membrane components between the two domains. Using βIV-spectrin knockout mice, we demonstrate the involvement of this cytoskeletal protein in the formation of a barrier that selectively blocks lateral mobility of L1 cell adhesion molecule (L1CAM) at the axon initial segment of hippocampal neurons. We also show that the βIV-spectrin-based barrier is required for the axon-specific distribution of L1CAM both in vitro and in vivo. The barrier activity against L1CAM may depend on direct interactions of L1CAM with ankyrinG, a protein binding to βIV-spectrin, rather than on steric hindrance by other transmembrane proteins clustered at the axon initial segment. Our results highlight the role of βIV-spectrin and ankyrinG as critical components of a selective barrier against L1CAM.

Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume34
Issue number3
DOIs
Publication statusPublished - 2007 Mar
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

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