βIV-spectrin forms a diffusion barrier against L1CAM at the axon initial segment

Kazunari Nishimura, Hiroki Akiyama, Masayuki Komada, Hiroyuki Kamiguchi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Axonal and somatodendritic plasma membranes of polarized neurons express distinct sets of functional molecules. It is known that the neuronal polarity can be maintained by a barrier that impedes diffusional mixing of membrane components between the two domains. Using βIV-spectrin knockout mice, we demonstrate the involvement of this cytoskeletal protein in the formation of a barrier that selectively blocks lateral mobility of L1 cell adhesion molecule (L1CAM) at the axon initial segment of hippocampal neurons. We also show that the βIV-spectrin-based barrier is required for the axon-specific distribution of L1CAM both in vitro and in vivo. The barrier activity against L1CAM may depend on direct interactions of L1CAM with ankyrinG, a protein binding to βIV-spectrin, rather than on steric hindrance by other transmembrane proteins clustered at the axon initial segment. Our results highlight the role of βIV-spectrin and ankyrinG as critical components of a selective barrier against L1CAM.

Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume34
Issue number3
DOIs
Publication statusPublished - 2007 Mar
Externally publishedYes

Fingerprint

Neural Cell Adhesion Molecule L1
Spectrin
Neurons
Cytoskeletal Proteins
Protein Binding
Knockout Mice
Axons
Cell Membrane
Axon Initial Segment
Membranes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

βIV-spectrin forms a diffusion barrier against L1CAM at the axon initial segment. / Nishimura, Kazunari; Akiyama, Hiroki; Komada, Masayuki; Kamiguchi, Hiroyuki.

In: Molecular and Cellular Neuroscience, Vol. 34, No. 3, 03.2007, p. 422-430.

Research output: Contribution to journalArticle

Nishimura, Kazunari ; Akiyama, Hiroki ; Komada, Masayuki ; Kamiguchi, Hiroyuki. / βIV-spectrin forms a diffusion barrier against L1CAM at the axon initial segment. In: Molecular and Cellular Neuroscience. 2007 ; Vol. 34, No. 3. pp. 422-430.
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