γ-Tubulin complex-mediated anchoring of spindle microtubules to spindle-pole bodies requires Msd1 in fission yeast

Mika Toya, Masamitsu Sato, Uta Haselmann, Kazuhide Asakawa, Damian Brunner, Claude Antony, Takashi Toda

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The anchoring of microtubules to subcellular structures is critical for cell polarity and motility. Although the process of anchoring cytoplasmic microtubules to the centrosome has been studied in some detail1-4, it is not known how spindle microtubules are anchored to the mitotic centrosome and, particularly, whether anchoring and nucleation of mitotic spindles are functionally separate. Here, we show that a fission yeast coiled-coil protein, Msd1, is required for anchoring the minus end of spindle microtubules to the centrosome equivalent, the spindle-pole body (SPB). msd1 deletion causes spindle microtubules to abnormally extend beyond SPBs, which results in chromosome missegregation. Importantly, this protruding spindle is phenocopied by the amino-terminal deletion mutant of Alp4, a component of the γ-tubulin complex5 (γ-TuC), which lacks the potential Msd1-interacting domain. We propose that Msd1 interacts with γ-TuC, thereby specifically anchoring the minus end of microtubules to SPBs without affecting microtubule nucleation.

Original languageEnglish
Pages (from-to)646-653
Number of pages8
JournalNature Cell Biology
Volume9
Issue number6
DOIs
Publication statusPublished - 2007 Jun
Externally publishedYes

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Spindle Pole Bodies
Schizosaccharomyces
Tubulin
Microtubules
Centrosome
Cell Polarity
Spindle Apparatus
Cell Movement
Chromosomes

ASJC Scopus subject areas

  • Cell Biology

Cite this

γ-Tubulin complex-mediated anchoring of spindle microtubules to spindle-pole bodies requires Msd1 in fission yeast. / Toya, Mika; Sato, Masamitsu; Haselmann, Uta; Asakawa, Kazuhide; Brunner, Damian; Antony, Claude; Toda, Takashi.

In: Nature Cell Biology, Vol. 9, No. 6, 06.2007, p. 646-653.

Research output: Contribution to journalArticle

Toya, Mika ; Sato, Masamitsu ; Haselmann, Uta ; Asakawa, Kazuhide ; Brunner, Damian ; Antony, Claude ; Toda, Takashi. / γ-Tubulin complex-mediated anchoring of spindle microtubules to spindle-pole bodies requires Msd1 in fission yeast. In: Nature Cell Biology. 2007 ; Vol. 9, No. 6. pp. 646-653.
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