1064 nm deep near-infrared (NIR) excited raman microspectroscopy for studying photolabile organisms

Masahiro Ando; Miwa Sugiura; Hidenori Hayashi; Hiro O. Hamaguchi

doi:10.1366/10-06196

1064 nm deep near-infrared (NIR) excited raman microspectroscopy for studying photolabile organisms

Masahiro Ando, Miwa Sugiura, Hidenori Hayashi, Hiro O. Hamaguchi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

We have constructed a 1064 nm deep near-infrared (NIR) excited multichannel Raman microspectrometer using an InP/InGaAsP multichannel detector. This microspectrometer achieves high sensitivity suitable for in vivo measurements of single living cells with lateral resolution of 0.7 μm and depth resolution of 3.1 μm. It has been applied to the structural analysis of living cyanobacterial cells, well-known model organisms for photosynthesis research, which are too photolabile to be measured with visible laser excitation. High signal-to-noise ratio (S/N) Raman spectra have been obtained from carotenoid, chlorophyll a, and phycocyanin in a single living cyanobacterial cell with no appreciable interference from autofluorescence or photodamage. Sub-micrometer mapping of Raman intensities provides clear distribution images of the three pigments inside the cell.

Original language	English
Pages (from-to)	488-492
Number of pages	5
Journal	Applied Spectroscopy
Volume	65
Issue number	5
DOIs	https://doi.org/10.1366/10-06196
Publication status	Published - 2011 May
Externally published	Yes

Keywords

1064 nm excitation
Cyanobacteria
Microspectroscopy
Multichannel raman spectroscopy
Near-infrared raman spectroscopy
Thermosynechococcus elongatus

ASJC Scopus subject areas

Instrumentation
Spectroscopy

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title = "1064 nm deep near-infrared (NIR) excited raman microspectroscopy for studying photolabile organisms",

abstract = "We have constructed a 1064 nm deep near-infrared (NIR) excited multichannel Raman microspectrometer using an InP/InGaAsP multichannel detector. This microspectrometer achieves high sensitivity suitable for in vivo measurements of single living cells with lateral resolution of 0.7 μm and depth resolution of 3.1 μm. It has been applied to the structural analysis of living cyanobacterial cells, well-known model organisms for photosynthesis research, which are too photolabile to be measured with visible laser excitation. High signal-to-noise ratio (S/N) Raman spectra have been obtained from carotenoid, chlorophyll a, and phycocyanin in a single living cyanobacterial cell with no appreciable interference from autofluorescence or photodamage. Sub-micrometer mapping of Raman intensities provides clear distribution images of the three pigments inside the cell.",

keywords = "1064 nm excitation, Cyanobacteria, Microspectroscopy, Multichannel raman spectroscopy, Near-infrared raman spectroscopy, Thermosynechococcus elongatus",

author = "Masahiro Ando and Miwa Sugiura and Hidenori Hayashi and Hamaguchi, {Hiro O.}",

year = "2011",

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doi = "10.1366/10-06196",

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AU - Ando, Masahiro

AU - Sugiura, Miwa

AU - Hayashi, Hidenori

AU - Hamaguchi, Hiro O.

PY - 2011/5

Y1 - 2011/5

N2 - We have constructed a 1064 nm deep near-infrared (NIR) excited multichannel Raman microspectrometer using an InP/InGaAsP multichannel detector. This microspectrometer achieves high sensitivity suitable for in vivo measurements of single living cells with lateral resolution of 0.7 μm and depth resolution of 3.1 μm. It has been applied to the structural analysis of living cyanobacterial cells, well-known model organisms for photosynthesis research, which are too photolabile to be measured with visible laser excitation. High signal-to-noise ratio (S/N) Raman spectra have been obtained from carotenoid, chlorophyll a, and phycocyanin in a single living cyanobacterial cell with no appreciable interference from autofluorescence or photodamage. Sub-micrometer mapping of Raman intensities provides clear distribution images of the three pigments inside the cell.

AB - We have constructed a 1064 nm deep near-infrared (NIR) excited multichannel Raman microspectrometer using an InP/InGaAsP multichannel detector. This microspectrometer achieves high sensitivity suitable for in vivo measurements of single living cells with lateral resolution of 0.7 μm and depth resolution of 3.1 μm. It has been applied to the structural analysis of living cyanobacterial cells, well-known model organisms for photosynthesis research, which are too photolabile to be measured with visible laser excitation. High signal-to-noise ratio (S/N) Raman spectra have been obtained from carotenoid, chlorophyll a, and phycocyanin in a single living cyanobacterial cell with no appreciable interference from autofluorescence or photodamage. Sub-micrometer mapping of Raman intensities provides clear distribution images of the three pigments inside the cell.

KW - 1064 nm excitation

KW - Cyanobacteria

KW - Microspectroscopy

KW - Multichannel raman spectroscopy

KW - Near-infrared raman spectroscopy

KW - Thermosynechococcus elongatus

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ER -

1064 nm deep near-infrared (NIR) excited raman microspectroscopy for studying photolabile organisms

Abstract

Keywords

ASJC Scopus subject areas

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