We successfully developed the Quantum Dot (QD) light source in the T-and O-band (Thousand-band: 1.000-1.260 μm and O-band: 1.260-1.360 μm).We used the optical gain chip properties of a single InAs/InGaAs QD to develop a 1.3 μm waveband QD optical frequency comb laser (QD-CML) as the novel functional wavelength divisio nmultiplexing (WDM) light source. The QD-CML can be used for stable generation of multiple-wavelength peaks that the QD-CML can be selected and controlled using the optical interference of the etalon filter. We used holey fiber (HF) transmission line to successfully demonstrate an O-band photonic transport system. In this experiment, each single peak could be clearly selected in the wavelength range the 1286-to 1302 nm. An error-free data transmission of the 10-Gb/s signal with stable multiple-wavelength channels that were generated from a newly developed 1.3 μm waveband multiple-wavelength quantum dot light source was achieved over the low-loss HF that was 8-km long. We suggest that the low cross-talk for a data transmission can be achieved using the multiple-wavelength peaks from the QD light source. Based on these experimental results, it is clear that the generation of multiple-wavelength peaks in the 1.3 μm waveband can be successfully achieved using from the multiple-wavelength QD light source, which acts as a single QD optical gain device.