16s rRNA-targeted identification of cyanobacterial genera using oligonucleotide-probes immobilized on bacterial magnetic particles

Tadashi Matsunaga, Haruko Takeyama, Hideki Nakayama

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

16S rRNA-targeted identification of cyanobacterial genera, Anabaena, Microcystis, Nostoc, Oscillatoria, Synechococcus was developed using bacterial magnetic particles (BMPs). 16S rRNA-targeted capture probes designed from the genus specific region of the 16S rRNA sequence were immobilized on BMPs. Identification of cyanobacteria was performed by a sandwich hybridization using the capture probes - BMP conjugates and a digoxigenin (DIG)-labeled detector probe complementary to the highly conserved 16S rRNA sequence for cyanobacteria. The luminescence intensity of the probe/target-BMP hybrids was measured after reaction with alkaline phosphatase conjugated anti-DIG antibody. Five species of cyanobacteria from five different genera were successfully discriminated using this magnetic capture system.

Original languageEnglish
Pages (from-to)389-394
Number of pages6
JournalJournal of Applied Phycology
Volume13
Issue number4
DOIs
Publication statusPublished - 2001 Oct 16
Externally publishedYes

Keywords

  • 16S rRNA
  • Bacterial magnetic particles(BMPs)
  • Cyanobacteria
  • Identification
  • Magnetic-capture hybridization

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

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