To detect phosphatidylinositol (PI) 3-kinases with high sensitivity, we designed and prepared a radiolabeled derivative of wortmannin (1), a potent inhibitor of PI 3-kinases. The synthesized derivative, 17ß-hydroxy-16α-[125I]iodowortmannin (7), showed an IC50 that was 100-fold higher than that of wortmannin itself and which bound to catalytic subunits of PI 3-kinases.
|Number of pages||3|
|Journal||Chemical and Pharmaceutical Bulletin|
|Publication status||Published - 1995|
- PI 3-kinase
- signal transduction
ASJC Scopus subject areas
- Drug Discovery