17β-Hydroxy-16α-[125I]iodowortmannin, a sensitive labeling agent for PI 3-kinases

S. Honzawa, M. Nakada, H. Kurosu, O. Hazeki, T. Katada, Masahisa Nakada

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    To detect phosphatidylinositol (PI) 3-kinases with high sensitivity, we designed and prepared a radiolabeled derivative of wortmannin (1), a potent inhibitor of PI 3-kinases. The synthesized derivative, 17β-hydroxy-16α- [125I]iodowortmannin (7), showed an IC50 that was 100-fold higher than that of wortmannin itself and which bound to catalytic subunits of PI 3- kinases.

    Original languageEnglish
    Pages (from-to)2276-2278
    Number of pages3
    JournalChemical and Pharmaceutical Bulletin
    Volume43
    Issue number12
    Publication statusPublished - 1995

    Fingerprint

    Phosphatidylinositol 3-Kinases
    Labeling
    Derivatives
    Inhibitory Concentration 50
    Catalytic Domain
    17-hydroxy-16-iodowortmannin
    wortmannin

    Keywords

    • PI 3-kinase
    • radiolabeling
    • signal transduction
    • wortmannin

    ASJC Scopus subject areas

    • Chemistry(all)
    • Organic Chemistry
    • Drug Discovery
    • Pharmacology

    Cite this

    Honzawa, S., Nakada, M., Kurosu, H., Hazeki, O., Katada, T., & Nakada, M. (1995). 17β-Hydroxy-16α-[125I]iodowortmannin, a sensitive labeling agent for PI 3-kinases. Chemical and Pharmaceutical Bulletin, 43(12), 2276-2278.

    17β-Hydroxy-16α-[125I]iodowortmannin, a sensitive labeling agent for PI 3-kinases. / Honzawa, S.; Nakada, M.; Kurosu, H.; Hazeki, O.; Katada, T.; Nakada, Masahisa.

    In: Chemical and Pharmaceutical Bulletin, Vol. 43, No. 12, 1995, p. 2276-2278.

    Research output: Contribution to journalArticle

    Honzawa, S, Nakada, M, Kurosu, H, Hazeki, O, Katada, T & Nakada, M 1995, '17β-Hydroxy-16α-[125I]iodowortmannin, a sensitive labeling agent for PI 3-kinases', Chemical and Pharmaceutical Bulletin, vol. 43, no. 12, pp. 2276-2278.
    Honzawa, S. ; Nakada, M. ; Kurosu, H. ; Hazeki, O. ; Katada, T. ; Nakada, Masahisa. / 17β-Hydroxy-16α-[125I]iodowortmannin, a sensitive labeling agent for PI 3-kinases. In: Chemical and Pharmaceutical Bulletin. 1995 ; Vol. 43, No. 12. pp. 2276-2278.
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    AU - Katada, T.

    AU - Nakada, Masahisa

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