1a-Docosahexaenoyl mitomycin C: A novel inhibitor of protein tyrosine kinase

Mayumi Shikano, Kenjiro Onimura, Yoshihisa Fukai, Makoto Hori, Hidesuke Fukazawa, Satoshi Mizuno, Kazunaga Yazawa, Yoshimasa Uehara

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A series of derivatives of mitomycin C conjugated with various fatty acids at position la was synthesized and the effect of these compounds on protein kinase activities was evaluated. 1a-Docosahexaenoyl mitomycin C (DMMC) selectively inhibited protein tyrosine kinase (PTK) activity in the postnuclear fraction of v-src-transformed NIH 3T3 cells although neither derivatives conjugated with other fatty acids or docosahexaenoic acid or mitomycin C did not. DMMC inhibited the activity of calmodulin-dependent kinase III and protein kinase A very weakly, and only barely affected protein kinase C activity. DMMC also attenuated autophosphorylation of immunoprecipitated p60(v-)src irreversibly. The addition of thiol compounds to the reaction mixture reversed the inhibition by DMMC, suggesting that some thiol moiety of PTK protein might be involved. DMMC also inhibited kinase activity of p210(bcr-abl) immunoprecipitated from the lysate of K562 cells. These results indicate that DMMC is a novel inhibitor of PTK.

Original languageEnglish
Pages (from-to)858-863
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume248
Issue number3
DOIs
Publication statusPublished - 1998 Jul 30
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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