31. Length Regulation of Thin Filaments without Nebulin

Kenji Yasuda, Hideaki Fujita, Yasutake Fujiki, Shinichi Ishiwata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

: Recent work has suggested that the length of thin filaments in skeletal muscles is determined by a protein ruler, nebulin, located along the long axis of thin filaments. To examine the function of nebulin, the length distribution of the thin filaments was investigated by staining actin filaments with fluorescent rhodamine-phalloidin in rabbit cardiac muscles, which do not contain nebulin, and in skeletal muscles, which contain nebulin, by laser scanning confocal microscopy. The microscopic observation showed a difference in staining patterns between cardiac and skeletal muscle fibers when the staining was done without chemical fixation. This suggests that nebulin suppresses the attachment of phalloidin to actin filaments. Analysis of fluorescence distribution showed that the length deviation of thin filaments in the cardiac muscle was as small as that in the skeletal muscle. This indicates that the length of the thin filaments is regulated even without nebulin.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume70
Issue number9
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

filaments
myocardium
skeletal muscle
microfilaments
staining
muscles
Myocardium
Skeletal Muscle
Staining and Labeling
Actin Cytoskeleton
confocal laser scanning microscopy
Phalloidine
muscle fibers
rabbits
Skeletal Muscle Fibers
fluorescence
Confocal Microscopy
rhodamine
nebulin
attachment

Keywords

  • Cardiac muscle
  • Laser scanning confocal microscopy
  • Nebulin
  • Rhodamine-phalloidin
  • Skeletal muscle
  • Thin filaments

ASJC Scopus subject areas

  • General

Cite this

31. Length Regulation of Thin Filaments without Nebulin. / Yasuda, Kenji; Fujita, Hideaki; Fujiki, Yasutake; Ishiwata, Shinichi.

In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences, Vol. 70, No. 9, 1994, p. 151-156.

Research output: Contribution to journalArticle

Yasuda, Kenji ; Fujita, Hideaki ; Fujiki, Yasutake ; Ishiwata, Shinichi. / 31. Length Regulation of Thin Filaments without Nebulin. In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences. 1994 ; Vol. 70, No. 9. pp. 151-156.
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