3D reconstruction of internal structure of animal body using near-infrared light

Trung Nghia Tran, Kohei Yamamoto, Takeshi Namita, Yuji Kato, Koichi Shimizu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To realize three-dimensional (3D) optical imaging of the internal structure of animal body, we have developed a new technique to reconstruct CT images from two-dimensional (2D) transillumination images. In transillumination imaging, the image is blurred due to the strong scattering in the tissue. We had developed a scattering suppression technique using the point spread function (PSF) for a fluorescent light source in the body. In this study, we have newly proposed a technique to apply this PSF for a light source to the image of unknown light-absorbing structure. The effectiveness of the proposed technique was examined in the experiments with a model phantom and a mouse. In the phantom experiment, the absorbers were placed in the tissue-equivalent medium to simulate the light-absorbing organs in mouse body. Near-infrared light was illuminated from one side of the phantom and the image was recorded with CMOS camera from another side. Using the proposed techniques, the scattering effect was efficiently suppressed and the absorbing structure can be visualized in the 2D transillumination image. Using the 2D images obtained in many different orientations, we could reconstruct the 3D image. In the mouse experiment, an anesthetized mouse was held in an acrylic cylindrical holder. We can visualize the internal organs such as kidneys through mouse's abdomen using the proposed technique. The 3D image of the kidneys and a part of the liver were reconstructed. Through these experimental studies, the feasibility of practical 3D imaging of the internal light-absorbing structure of a small animal was verified.

Original languageEnglish
Title of host publicationBiomedical Applications of Light Scattering VIII
PublisherSPIE
Volume8952
ISBN (Print)9780819498656
DOIs
Publication statusPublished - 2014
Externally publishedYes
EventBiomedical Applications of Light Scattering VIII - San Francisco, CA, United States
Duration: 2014 Feb 12014 Feb 3

Other

OtherBiomedical Applications of Light Scattering VIII
CountryUnited States
CitySan Francisco, CA
Period14/2/114/2/3

Fingerprint

Animal Structures
Transillumination
animals
Animals
Infrared radiation
Light
Optical transfer function
Scattering
mice
Imaging techniques
Light sources
Tissue
Experiments
kidneys
Liver
point spread functions
Acrylics
Kidney
organs
Optical Imaging

Keywords

  • 3D optical imaging
  • 3D reconstruction
  • Bio-imaging
  • Diffuse optical tomography
  • Point spread function
  • Scattering suppression
  • Small animal imaging
  • Transillumination image

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Tran, T. N., Yamamoto, K., Namita, T., Kato, Y., & Shimizu, K. (2014). 3D reconstruction of internal structure of animal body using near-infrared light. In Biomedical Applications of Light Scattering VIII (Vol. 8952). [89521A] SPIE. https://doi.org/10.1117/12.2038474

3D reconstruction of internal structure of animal body using near-infrared light. / Tran, Trung Nghia; Yamamoto, Kohei; Namita, Takeshi; Kato, Yuji; Shimizu, Koichi.

Biomedical Applications of Light Scattering VIII. Vol. 8952 SPIE, 2014. 89521A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tran, TN, Yamamoto, K, Namita, T, Kato, Y & Shimizu, K 2014, 3D reconstruction of internal structure of animal body using near-infrared light. in Biomedical Applications of Light Scattering VIII. vol. 8952, 89521A, SPIE, Biomedical Applications of Light Scattering VIII, San Francisco, CA, United States, 14/2/1. https://doi.org/10.1117/12.2038474
Tran TN, Yamamoto K, Namita T, Kato Y, Shimizu K. 3D reconstruction of internal structure of animal body using near-infrared light. In Biomedical Applications of Light Scattering VIII. Vol. 8952. SPIE. 2014. 89521A https://doi.org/10.1117/12.2038474
Tran, Trung Nghia ; Yamamoto, Kohei ; Namita, Takeshi ; Kato, Yuji ; Shimizu, Koichi. / 3D reconstruction of internal structure of animal body using near-infrared light. Biomedical Applications of Light Scattering VIII. Vol. 8952 SPIE, 2014.
@inproceedings{7a711bfa80084869abd88a9cc333e856,
title = "3D reconstruction of internal structure of animal body using near-infrared light",
abstract = "To realize three-dimensional (3D) optical imaging of the internal structure of animal body, we have developed a new technique to reconstruct CT images from two-dimensional (2D) transillumination images. In transillumination imaging, the image is blurred due to the strong scattering in the tissue. We had developed a scattering suppression technique using the point spread function (PSF) for a fluorescent light source in the body. In this study, we have newly proposed a technique to apply this PSF for a light source to the image of unknown light-absorbing structure. The effectiveness of the proposed technique was examined in the experiments with a model phantom and a mouse. In the phantom experiment, the absorbers were placed in the tissue-equivalent medium to simulate the light-absorbing organs in mouse body. Near-infrared light was illuminated from one side of the phantom and the image was recorded with CMOS camera from another side. Using the proposed techniques, the scattering effect was efficiently suppressed and the absorbing structure can be visualized in the 2D transillumination image. Using the 2D images obtained in many different orientations, we could reconstruct the 3D image. In the mouse experiment, an anesthetized mouse was held in an acrylic cylindrical holder. We can visualize the internal organs such as kidneys through mouse's abdomen using the proposed technique. The 3D image of the kidneys and a part of the liver were reconstructed. Through these experimental studies, the feasibility of practical 3D imaging of the internal light-absorbing structure of a small animal was verified.",
keywords = "3D optical imaging, 3D reconstruction, Bio-imaging, Diffuse optical tomography, Point spread function, Scattering suppression, Small animal imaging, Transillumination image",
author = "Tran, {Trung Nghia} and Kohei Yamamoto and Takeshi Namita and Yuji Kato and Koichi Shimizu",
year = "2014",
doi = "10.1117/12.2038474",
language = "English",
isbn = "9780819498656",
volume = "8952",
booktitle = "Biomedical Applications of Light Scattering VIII",
publisher = "SPIE",
address = "United States",

}

TY - GEN

T1 - 3D reconstruction of internal structure of animal body using near-infrared light

AU - Tran, Trung Nghia

AU - Yamamoto, Kohei

AU - Namita, Takeshi

AU - Kato, Yuji

AU - Shimizu, Koichi

PY - 2014

Y1 - 2014

N2 - To realize three-dimensional (3D) optical imaging of the internal structure of animal body, we have developed a new technique to reconstruct CT images from two-dimensional (2D) transillumination images. In transillumination imaging, the image is blurred due to the strong scattering in the tissue. We had developed a scattering suppression technique using the point spread function (PSF) for a fluorescent light source in the body. In this study, we have newly proposed a technique to apply this PSF for a light source to the image of unknown light-absorbing structure. The effectiveness of the proposed technique was examined in the experiments with a model phantom and a mouse. In the phantom experiment, the absorbers were placed in the tissue-equivalent medium to simulate the light-absorbing organs in mouse body. Near-infrared light was illuminated from one side of the phantom and the image was recorded with CMOS camera from another side. Using the proposed techniques, the scattering effect was efficiently suppressed and the absorbing structure can be visualized in the 2D transillumination image. Using the 2D images obtained in many different orientations, we could reconstruct the 3D image. In the mouse experiment, an anesthetized mouse was held in an acrylic cylindrical holder. We can visualize the internal organs such as kidneys through mouse's abdomen using the proposed technique. The 3D image of the kidneys and a part of the liver were reconstructed. Through these experimental studies, the feasibility of practical 3D imaging of the internal light-absorbing structure of a small animal was verified.

AB - To realize three-dimensional (3D) optical imaging of the internal structure of animal body, we have developed a new technique to reconstruct CT images from two-dimensional (2D) transillumination images. In transillumination imaging, the image is blurred due to the strong scattering in the tissue. We had developed a scattering suppression technique using the point spread function (PSF) for a fluorescent light source in the body. In this study, we have newly proposed a technique to apply this PSF for a light source to the image of unknown light-absorbing structure. The effectiveness of the proposed technique was examined in the experiments with a model phantom and a mouse. In the phantom experiment, the absorbers were placed in the tissue-equivalent medium to simulate the light-absorbing organs in mouse body. Near-infrared light was illuminated from one side of the phantom and the image was recorded with CMOS camera from another side. Using the proposed techniques, the scattering effect was efficiently suppressed and the absorbing structure can be visualized in the 2D transillumination image. Using the 2D images obtained in many different orientations, we could reconstruct the 3D image. In the mouse experiment, an anesthetized mouse was held in an acrylic cylindrical holder. We can visualize the internal organs such as kidneys through mouse's abdomen using the proposed technique. The 3D image of the kidneys and a part of the liver were reconstructed. Through these experimental studies, the feasibility of practical 3D imaging of the internal light-absorbing structure of a small animal was verified.

KW - 3D optical imaging

KW - 3D reconstruction

KW - Bio-imaging

KW - Diffuse optical tomography

KW - Point spread function

KW - Scattering suppression

KW - Small animal imaging

KW - Transillumination image

UR - http://www.scopus.com/inward/record.url?scp=84897438503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897438503&partnerID=8YFLogxK

U2 - 10.1117/12.2038474

DO - 10.1117/12.2038474

M3 - Conference contribution

SN - 9780819498656

VL - 8952

BT - Biomedical Applications of Light Scattering VIII

PB - SPIE

ER -