5-Aminolevulinic acid enhances cancer radiotherapy in a mouse tumor model

Junko Takahashi*, Masaki Misawa, Mami Murakami, Takashi Mori, Kazuki Nomura, Hitoshi Iwahashi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

5-Aminolevulinic acid (ALA) is a photosensitizer used in photodynamic therapy (PDT) because it causes preferential accumulation of protoporphyrin IX (PpIX) in tumor cells, where it forms singlet oxygen upon light irradiation and kills the tumor cells. Our previous study demonstrated that PpIX enhances generation of reactive oxygen species by physicochemical interaction with X-rays. We investigated the effect of ALA administration with X-ray irradiation of mouse B16-BL6 melanoma cells in vitro and in vivo. ALA facilitates PpIX accumulation in tumor cells and enhances ROS generation in vitro. Tumor suppression significantly improved in animals treated with fractionated doses of radiation (3 Gy × 10; total, 30 Gy) with local administration of 50 mg/kg ALA at 24 h prior to fractional irradiation. These results suggest ALA may improve the efficacy of cancer radiotherapy by acting as a radiomediator.

Original languageEnglish
Article number602
Pages (from-to)1-6
Number of pages6
JournalSpringerPlus
Volume2
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • 5-aminolevulinic acid
  • Cancer
  • Protoporphyrin
  • Radiotherapy
  • X-ray

ASJC Scopus subject areas

  • General

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