7-methylguanosine at the anticodon wobble position of squid mitochondrial tRNA(Ser)GCU: Molecular basis for assignment of AGA/AGG codons as serine in invertebrate mitochondria

Kozo Tomita, Takuya Ueda, Kimitsuna Watanabe

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In mitochondria of the squid, Loligo bleekeri, both the AGA and AGG codons are considered to correspond to serine instead of arginine as in the universal genetic code, and its genome encodes a single tRNA(Scr) gene with the anticodon GCT. Therefore, this gene product, tRNA(Ser)GCU, should be able to translate all four AGN (N; U, C, A, and G) codons as serine. To elucidate this recognition mechanism, the tRNA(Ser)GCU was isolated from squid liver and its complete nucleotide sequence determined. The tRNA(Ser)GCU was found to possess 7-methylguanosine (m7G) at the wobble position of the anticodon. This suggests that in the squid mitochondrial system, tRNA(Ser)GCU with the anticodon m7GCU can recognize not only the usual serine codons AGU and AGC, but also the unusual serine codons AGA and AGG, as in the case of starfish mitochondria (Matsuyama et al., J. Biol. Chem. 273 (1988) 3363-3368).

Original languageEnglish
Pages (from-to)78-82
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1399
Issue number1
DOIs
Publication statusPublished - 1998 Jul 30
Externally publishedYes

Fingerprint

Anticodon
Decapodiformes
Mitochondria
Invertebrates
Transfer RNA
Codon
Serine
Genes
Loligo
Starfish
Genetic Code
7-methylguanosine
Liver
Arginine
Nucleotides
Genome

Keywords

  • (Squid, Loligo bleekeri)
  • 7- Methylguanosine
  • Decoding of AGA and AGG codons
  • Mitochondrion
  • TRNA(Ser)GCU

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

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abstract = "In mitochondria of the squid, Loligo bleekeri, both the AGA and AGG codons are considered to correspond to serine instead of arginine as in the universal genetic code, and its genome encodes a single tRNA(Scr) gene with the anticodon GCT. Therefore, this gene product, tRNA(Ser)GCU, should be able to translate all four AGN (N; U, C, A, and G) codons as serine. To elucidate this recognition mechanism, the tRNA(Ser)GCU was isolated from squid liver and its complete nucleotide sequence determined. The tRNA(Ser)GCU was found to possess 7-methylguanosine (m7G) at the wobble position of the anticodon. This suggests that in the squid mitochondrial system, tRNA(Ser)GCU with the anticodon m7GCU can recognize not only the usual serine codons AGU and AGC, but also the unusual serine codons AGA and AGG, as in the case of starfish mitochondria (Matsuyama et al., J. Biol. Chem. 273 (1988) 3363-3368).",
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T1 - 7-methylguanosine at the anticodon wobble position of squid mitochondrial tRNA(Ser)GCU

T2 - Molecular basis for assignment of AGA/AGG codons as serine in invertebrate mitochondria

AU - Tomita, Kozo

AU - Ueda, Takuya

AU - Watanabe, Kimitsuna

PY - 1998/7/30

Y1 - 1998/7/30

N2 - In mitochondria of the squid, Loligo bleekeri, both the AGA and AGG codons are considered to correspond to serine instead of arginine as in the universal genetic code, and its genome encodes a single tRNA(Scr) gene with the anticodon GCT. Therefore, this gene product, tRNA(Ser)GCU, should be able to translate all four AGN (N; U, C, A, and G) codons as serine. To elucidate this recognition mechanism, the tRNA(Ser)GCU was isolated from squid liver and its complete nucleotide sequence determined. The tRNA(Ser)GCU was found to possess 7-methylguanosine (m7G) at the wobble position of the anticodon. This suggests that in the squid mitochondrial system, tRNA(Ser)GCU with the anticodon m7GCU can recognize not only the usual serine codons AGU and AGC, but also the unusual serine codons AGA and AGG, as in the case of starfish mitochondria (Matsuyama et al., J. Biol. Chem. 273 (1988) 3363-3368).

AB - In mitochondria of the squid, Loligo bleekeri, both the AGA and AGG codons are considered to correspond to serine instead of arginine as in the universal genetic code, and its genome encodes a single tRNA(Scr) gene with the anticodon GCT. Therefore, this gene product, tRNA(Ser)GCU, should be able to translate all four AGN (N; U, C, A, and G) codons as serine. To elucidate this recognition mechanism, the tRNA(Ser)GCU was isolated from squid liver and its complete nucleotide sequence determined. The tRNA(Ser)GCU was found to possess 7-methylguanosine (m7G) at the wobble position of the anticodon. This suggests that in the squid mitochondrial system, tRNA(Ser)GCU with the anticodon m7GCU can recognize not only the usual serine codons AGU and AGC, but also the unusual serine codons AGA and AGG, as in the case of starfish mitochondria (Matsuyama et al., J. Biol. Chem. 273 (1988) 3363-3368).

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