A 210-kb segment of tandem repeats and retroelements located between imprinted subdomains of mouse distal chromosome 7

Hisao Shirohzu, Takaaki Yokomine, Chiyoko Sato, Reiko Kato, Atsushi Toyoda, Wahyu Purbowasito, Chikako Suda, Tsunehiro Mukai, Masahira Hattori, Katsuzumi Okumura, Yoshiyuki Sakaki, Hiroyuki Sasaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Mammalian genes subject to genomic imprinting often form clusters and are regulated by long-range mechanisms. The distal imprinted domain of mouse chromosome 7 is orthologous to the Beckwith-Wiedemann syndrome domain in human chromosome 11p15.5 and contains at least 13 imprinted genes. This domain consists of two subdomains, which are respectively regulated by an imprinting center. We here report the finished-quality sequence of a 0.6-Mb region encompassing the more centromeric subdomain. The sequence contains four imprinted genes (Ascl2/Mash2, Ins2, Igf2 and H19) and reveals previously unidentified CpG islands and tandem repeats, which may be features of imprinted genes. Most interestingly, a unique 210-kb segment consisting almost exclusively of tandem repeats and retroelements is identified. This segment, located between Th and Ins2, has features of heterochromatin-forming DNA and is highly methylated at CpG sites. The segment exhibits asynchronous replication on the parental chromosomes, a feature of the imprinted domains. We propose that this repeat segment could serve either as a boundary between the two subdomains or as a target for epigenetic chromatin modifications that regulate imprinting.

Original languageEnglish
Pages (from-to)325-334
Number of pages10
JournalDNA Research
Volume11
Issue number5
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Retroelements
Tandem Repeat Sequences
Chromosomes, Human, Pair 7
Genes
Beckwith-Wiedemann Syndrome
Genomic Imprinting
CpG Islands
Heterochromatin
Human Chromosomes
Epigenomics
Chromatin
Chromosomes
DNA

Keywords

  • DNA methylation
  • Genomic imprinting
  • Replication timing
  • Retroelement
  • Tandem repeat

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Shirohzu, H., Yokomine, T., Sato, C., Kato, R., Toyoda, A., Purbowasito, W., ... Sasaki, H. (2004). A 210-kb segment of tandem repeats and retroelements located between imprinted subdomains of mouse distal chromosome 7. DNA Research, 11(5), 325-334. https://doi.org/10.1093/dnares/11.5.325

A 210-kb segment of tandem repeats and retroelements located between imprinted subdomains of mouse distal chromosome 7. / Shirohzu, Hisao; Yokomine, Takaaki; Sato, Chiyoko; Kato, Reiko; Toyoda, Atsushi; Purbowasito, Wahyu; Suda, Chikako; Mukai, Tsunehiro; Hattori, Masahira; Okumura, Katsuzumi; Sakaki, Yoshiyuki; Sasaki, Hiroyuki.

In: DNA Research, Vol. 11, No. 5, 2004, p. 325-334.

Research output: Contribution to journalArticle

Shirohzu, H, Yokomine, T, Sato, C, Kato, R, Toyoda, A, Purbowasito, W, Suda, C, Mukai, T, Hattori, M, Okumura, K, Sakaki, Y & Sasaki, H 2004, 'A 210-kb segment of tandem repeats and retroelements located between imprinted subdomains of mouse distal chromosome 7', DNA Research, vol. 11, no. 5, pp. 325-334. https://doi.org/10.1093/dnares/11.5.325
Shirohzu, Hisao ; Yokomine, Takaaki ; Sato, Chiyoko ; Kato, Reiko ; Toyoda, Atsushi ; Purbowasito, Wahyu ; Suda, Chikako ; Mukai, Tsunehiro ; Hattori, Masahira ; Okumura, Katsuzumi ; Sakaki, Yoshiyuki ; Sasaki, Hiroyuki. / A 210-kb segment of tandem repeats and retroelements located between imprinted subdomains of mouse distal chromosome 7. In: DNA Research. 2004 ; Vol. 11, No. 5. pp. 325-334.
@article{124e5c0bb8b64a4eb619a4a600005325,
title = "A 210-kb segment of tandem repeats and retroelements located between imprinted subdomains of mouse distal chromosome 7",
abstract = "Mammalian genes subject to genomic imprinting often form clusters and are regulated by long-range mechanisms. The distal imprinted domain of mouse chromosome 7 is orthologous to the Beckwith-Wiedemann syndrome domain in human chromosome 11p15.5 and contains at least 13 imprinted genes. This domain consists of two subdomains, which are respectively regulated by an imprinting center. We here report the finished-quality sequence of a 0.6-Mb region encompassing the more centromeric subdomain. The sequence contains four imprinted genes (Ascl2/Mash2, Ins2, Igf2 and H19) and reveals previously unidentified CpG islands and tandem repeats, which may be features of imprinted genes. Most interestingly, a unique 210-kb segment consisting almost exclusively of tandem repeats and retroelements is identified. This segment, located between Th and Ins2, has features of heterochromatin-forming DNA and is highly methylated at CpG sites. The segment exhibits asynchronous replication on the parental chromosomes, a feature of the imprinted domains. We propose that this repeat segment could serve either as a boundary between the two subdomains or as a target for epigenetic chromatin modifications that regulate imprinting.",
keywords = "DNA methylation, Genomic imprinting, Replication timing, Retroelement, Tandem repeat",
author = "Hisao Shirohzu and Takaaki Yokomine and Chiyoko Sato and Reiko Kato and Atsushi Toyoda and Wahyu Purbowasito and Chikako Suda and Tsunehiro Mukai and Masahira Hattori and Katsuzumi Okumura and Yoshiyuki Sakaki and Hiroyuki Sasaki",
year = "2004",
doi = "10.1093/dnares/11.5.325",
language = "English",
volume = "11",
pages = "325--334",
journal = "DNA Research",
issn = "1340-2838",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - A 210-kb segment of tandem repeats and retroelements located between imprinted subdomains of mouse distal chromosome 7

AU - Shirohzu, Hisao

AU - Yokomine, Takaaki

AU - Sato, Chiyoko

AU - Kato, Reiko

AU - Toyoda, Atsushi

AU - Purbowasito, Wahyu

AU - Suda, Chikako

AU - Mukai, Tsunehiro

AU - Hattori, Masahira

AU - Okumura, Katsuzumi

AU - Sakaki, Yoshiyuki

AU - Sasaki, Hiroyuki

PY - 2004

Y1 - 2004

N2 - Mammalian genes subject to genomic imprinting often form clusters and are regulated by long-range mechanisms. The distal imprinted domain of mouse chromosome 7 is orthologous to the Beckwith-Wiedemann syndrome domain in human chromosome 11p15.5 and contains at least 13 imprinted genes. This domain consists of two subdomains, which are respectively regulated by an imprinting center. We here report the finished-quality sequence of a 0.6-Mb region encompassing the more centromeric subdomain. The sequence contains four imprinted genes (Ascl2/Mash2, Ins2, Igf2 and H19) and reveals previously unidentified CpG islands and tandem repeats, which may be features of imprinted genes. Most interestingly, a unique 210-kb segment consisting almost exclusively of tandem repeats and retroelements is identified. This segment, located between Th and Ins2, has features of heterochromatin-forming DNA and is highly methylated at CpG sites. The segment exhibits asynchronous replication on the parental chromosomes, a feature of the imprinted domains. We propose that this repeat segment could serve either as a boundary between the two subdomains or as a target for epigenetic chromatin modifications that regulate imprinting.

AB - Mammalian genes subject to genomic imprinting often form clusters and are regulated by long-range mechanisms. The distal imprinted domain of mouse chromosome 7 is orthologous to the Beckwith-Wiedemann syndrome domain in human chromosome 11p15.5 and contains at least 13 imprinted genes. This domain consists of two subdomains, which are respectively regulated by an imprinting center. We here report the finished-quality sequence of a 0.6-Mb region encompassing the more centromeric subdomain. The sequence contains four imprinted genes (Ascl2/Mash2, Ins2, Igf2 and H19) and reveals previously unidentified CpG islands and tandem repeats, which may be features of imprinted genes. Most interestingly, a unique 210-kb segment consisting almost exclusively of tandem repeats and retroelements is identified. This segment, located between Th and Ins2, has features of heterochromatin-forming DNA and is highly methylated at CpG sites. The segment exhibits asynchronous replication on the parental chromosomes, a feature of the imprinted domains. We propose that this repeat segment could serve either as a boundary between the two subdomains or as a target for epigenetic chromatin modifications that regulate imprinting.

KW - DNA methylation

KW - Genomic imprinting

KW - Replication timing

KW - Retroelement

KW - Tandem repeat

UR - http://www.scopus.com/inward/record.url?scp=19944423074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944423074&partnerID=8YFLogxK

U2 - 10.1093/dnares/11.5.325

DO - 10.1093/dnares/11.5.325

M3 - Article

VL - 11

SP - 325

EP - 334

JO - DNA Research

JF - DNA Research

SN - 1340-2838

IS - 5

ER -