A balanced diet is necessary for proper entrainment signals of the mouse liver clock

Akiko Hirao, Yu Tahara, Ichiro Kimura, Shigenobu Shibata

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Background: The peripheral circadian clock in mice is entrained not only by light-dark cycles but also by daily restricted feeding schedules. Behavioral and cell culture experiments suggest an increase in glucose level as a factor in such feeding-induced entrainment. For application of feeding-induced entrainment in humans, nutrient content and dietary variations should be considered. Principal Finding: To elucidate the food composition necessary for dietary entrainment, we examined whether complete or partial substitution of dietary nutrients affected phase shifts in liver clocks of mice. Compared with fasting mice or ad libitum fed mice, the liver bioluminescence rhythm advanced by 3-4 h on the middle day in Per2::luciferase knock-in mice that were administered a standard mouse diet, i.e. AIN-93M formula [0.6-0.85 g/10 g mouse BW] (composition: 14% casein, 47% cornstarch, 15% gelatinized cornstarch, 10% sugar, 4% soybean oil, and 10% other [fiber, vitamins, minerals, etc.]), for 2 days. When each nutrient was tested alone (100% nutrient), an insignificant weak phase advance was found to be induced by cornstarch and soybean oil, but almost no phase advance was induced by gelatinized cornstarch, high-amylose cornstarch, glucose, sucrose, or casein. A combination of glucose and casein without oil, vitamin, or fiber caused a significant phase advance. When cornstarch in AIN-93M was substituted with glucose, sucrose, fructose, polydextrose, high-amylose cornstarch, or gelatinized cornstarch, the amplitude of phase advance paralleled the increase in blood glucose concentration. Conclusions: Our results strongly suggest the following: (1) balanced diets containing carbohydrates/sugars and proteins are good for restricted feeding-induced entrainment of the peripheral circadian clock and (2) a balanced diet that increases blood glucose, but not by sugar alone, is suitable for entrainment. These findings may assist in the development of dietary recommendations for on-board meals served to air travelers and shift workers to reduce jet lag-like symptoms.

Original languageEnglish
Article numbere6909
JournalPLoS One
Volume4
Issue number9
DOIs
Publication statusPublished - 2009 Sep 7

Fingerprint

Nutrition
corn starch
Starch
Liver
Clocks
Diet
liver
mice
diet
Nutrients
Food
Caseins
Sugars
Glucose
casein
Amylose
Soybean Oil
Circadian Clocks
glucose
polydextrose

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A balanced diet is necessary for proper entrainment signals of the mouse liver clock. / Hirao, Akiko; Tahara, Yu; Kimura, Ichiro; Shibata, Shigenobu.

In: PLoS One, Vol. 4, No. 9, e6909, 07.09.2009.

Research output: Contribution to journalArticle

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