A catalytic immuno-reactor for the amperometric determination of human serum albumin

Isao Karube, Tadashi Matsunaga, Takeru Satoh, Shuichi Suzuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml-1.

Original languageEnglish
Pages (from-to)283-287
Number of pages5
JournalAnalytica Chimica Acta
Volume156
Issue numberC
DOIs
Publication statusPublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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