A catalytic immuno-reactor for the amperometric determination of human serum albumin

Isao Karube, Tadashi Matsunaga, Takeru Satoh, Shuichi Suzuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml-1.

Original languageEnglish
Pages (from-to)283-287
Number of pages5
JournalAnalytica Chimica Acta
Volume156
Issue numberC
DOIs
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

Hemin
Serum Albumin
antibody
serum
Hydrogen Peroxide
Antibodies
hydrogen peroxide
Immobilized Antibodies
Assays
Catalyst activity
antigen
Detectors
Decomposition
Antigens
assay
decomposition
reactor

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

A catalytic immuno-reactor for the amperometric determination of human serum albumin. / Karube, Isao; Matsunaga, Tadashi; Satoh, Takeru; Suzuki, Shuichi.

In: Analytica Chimica Acta, Vol. 156, No. C, 1984, p. 283-287.

Research output: Contribution to journalArticle

Karube, Isao ; Matsunaga, Tadashi ; Satoh, Takeru ; Suzuki, Shuichi. / A catalytic immuno-reactor for the amperometric determination of human serum albumin. In: Analytica Chimica Acta. 1984 ; Vol. 156, No. C. pp. 283-287.
@article{3d93c10a292541c2bdc09916752ae26a,
title = "A catalytic immuno-reactor for the amperometric determination of human serum albumin",
abstract = "A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9{\%} at an HSA concentration of 1 mg ml-1.",
author = "Isao Karube and Tadashi Matsunaga and Takeru Satoh and Shuichi Suzuki",
year = "1984",
doi = "10.1016/S0003-2670(00)85560-9",
language = "English",
volume = "156",
pages = "283--287",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "C",

}

TY - JOUR

T1 - A catalytic immuno-reactor for the amperometric determination of human serum albumin

AU - Karube, Isao

AU - Matsunaga, Tadashi

AU - Satoh, Takeru

AU - Suzuki, Shuichi

PY - 1984

Y1 - 1984

N2 - A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml-1.

AB - A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml-1.

UR - http://www.scopus.com/inward/record.url?scp=21244447461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21244447461&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(00)85560-9

DO - 10.1016/S0003-2670(00)85560-9

M3 - Article

AN - SCOPUS:21244447461

VL - 156

SP - 283

EP - 287

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - C

ER -