A cDNA encoding a new member of the rat placental lactogen family, PL-I mosaic (PL-Im)

M. Hirosawa, R. Miura, K. S. Min, N. Hattori, K. Shiota, T. Ogawa

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We have isolated a cDNA encoding a novel placental lactogen (PL), PL-I mosaic (PL-Im), by screening the cDNA library in λZAP of the mid-pregnant (day 12) rat placenta. The cDNA comprised an open reading frame of 687 bp encoding 229 amino acids, in which there were two putative N-glycosylation sites. Northern blot analysis showed that PL-Im mRNA was expressed specifically during mid-pregnancy (days 10 and 12) in the rat placenta. The cDNA was highly homologous with those of other rat PL family members; in particular, the homology among PL-Im, PL-I (mid-pregnancy-specific) and PL-Iv (late-pregnancy-specific) was over 90%. Interestingly, the nucleotide sequence of PL-Im cDNA was a mosaic of PL-I and PL-Iv and it did not possess its own particular sequence. As the genomic pattern determined using Southern blot analysis of PL-Im was distinct from that of PL-I and PL-Iv, the encoded area of PL-Im appears to be independent of those of PL-I and PL-Iv on the gene. In the dendrogram of the rat PL family constructed on the basis of the nucleotide sequence homologies, PL-Im was located between PL-Iv and PL-I in the process of molecular evolution. Therefore, PL-Im has a unique cDNA structure and may be a principal factor in the molecular evolution of PLs.

Original languageEnglish
Pages (from-to)387-397
Number of pages11
JournalEndocrine Journal
Volume41
Issue number4
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Placental Lactogen
Complementary DNA
Molecular Evolution
Pregnancy
Placenta
rat placental lactogen I
Sequence Homology
Southern Blotting
1-myristoyl-2-(12-N-(4-azido-2-nitrophenyl)aminododecanoyl)phosphatidylcholine
Gene Library
Glycosylation
Northern Blotting
Open Reading Frames
Amino Acids
Messenger RNA

Keywords

  • cDNA
  • cloning
  • placental lactogen I mosaic
  • rat placental lactogen

ASJC Scopus subject areas

  • Endocrinology

Cite this

Hirosawa, M., Miura, R., Min, K. S., Hattori, N., Shiota, K., & Ogawa, T. (1994). A cDNA encoding a new member of the rat placental lactogen family, PL-I mosaic (PL-Im). Endocrine Journal, 41(4), 387-397.

A cDNA encoding a new member of the rat placental lactogen family, PL-I mosaic (PL-Im). / Hirosawa, M.; Miura, R.; Min, K. S.; Hattori, N.; Shiota, K.; Ogawa, T.

In: Endocrine Journal, Vol. 41, No. 4, 1994, p. 387-397.

Research output: Contribution to journalArticle

Hirosawa, M, Miura, R, Min, KS, Hattori, N, Shiota, K & Ogawa, T 1994, 'A cDNA encoding a new member of the rat placental lactogen family, PL-I mosaic (PL-Im)', Endocrine Journal, vol. 41, no. 4, pp. 387-397.
Hirosawa M, Miura R, Min KS, Hattori N, Shiota K, Ogawa T. A cDNA encoding a new member of the rat placental lactogen family, PL-I mosaic (PL-Im). Endocrine Journal. 1994;41(4):387-397.
Hirosawa, M. ; Miura, R. ; Min, K. S. ; Hattori, N. ; Shiota, K. ; Ogawa, T. / A cDNA encoding a new member of the rat placental lactogen family, PL-I mosaic (PL-Im). In: Endocrine Journal. 1994 ; Vol. 41, No. 4. pp. 387-397.
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