A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells

Yusuke Yamamoto, Agnieszka Banas, Shigenori Murata, Madoka Ishikawa, Chun R. Lim, Takumi Teratani, Izuho Hatada, Kenichi Matsubara, Takashi Kato, Takahiro Ochiya

    Research output: Contribution to journalArticle

    64 Citations (Scopus)

    Abstract

    The specific features of the plasticity of adult stem cells are largely unknown. Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs). To identify the genes responsible for hepatic differentiation, we examined the gene expression profiles of AT-MSC-derived hepatocytes (AT-MSC-Hepa) using several microarray methods. The resulting sets of differentially expressed genes (1639 clones) were comprehensively analyzed to identify the pathways expressed in AT-MSC-Hepa. Clustering analysis revealed a striking similarity of gene clusters between AT-MSC-Hepa and the whole liver, indicating that AT-MSC-Hepa were similar to liver with regard to gene expression. Further analysis showed that enriched categories of genes and signaling pathways such as complementary activation and the blood clotting cascade in the AT-MSC-Hepa were relevant to liver-specific functions. Notably, decreases in Twist and Snail expression indicated that mesenchymal-to-epithelial transition occurred in the differentiation of AT-MSCs into hepatocytes. Our data show a similarity between AT-MSC-Hepa and the liver, suggesting that AT-MSCs are modulated by their environmental conditions, and that AT-MSC-Hepa may be useful in basic studies of liver function as well as in the development of stem cell-based therapy.

    Original languageEnglish
    Pages (from-to)1260-1273
    Number of pages14
    JournalFEBS Journal
    Volume275
    Issue number6
    DOIs
    Publication statusPublished - 2008 Mar

    Fingerprint

    Gene Expression Profiling
    Stem cells
    Mesenchymal Stromal Cells
    Adipose Tissue
    Signal Transduction
    Tissue
    Liver
    Genes
    Hepatocytes
    Gene expression
    Adult Stem Cells
    Epithelial-Mesenchymal Transition
    Snails
    Blood Coagulation
    Multigene Family
    Cell- and Tissue-Based Therapy
    Transcriptome
    Cluster Analysis
    Microarrays
    Stem Cells

    Keywords

    • Adipose tissue
    • Gene ontology
    • Hepatocyte differentiation
    • Mesenchymal stem cell
    • Microarray

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells. / Yamamoto, Yusuke; Banas, Agnieszka; Murata, Shigenori; Ishikawa, Madoka; Lim, Chun R.; Teratani, Takumi; Hatada, Izuho; Matsubara, Kenichi; Kato, Takashi; Ochiya, Takahiro.

    In: FEBS Journal, Vol. 275, No. 6, 03.2008, p. 1260-1273.

    Research output: Contribution to journalArticle

    Yamamoto, Y, Banas, A, Murata, S, Ishikawa, M, Lim, CR, Teratani, T, Hatada, I, Matsubara, K, Kato, T & Ochiya, T 2008, 'A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells', FEBS Journal, vol. 275, no. 6, pp. 1260-1273. https://doi.org/10.1111/j.1742-4658.2008.06287.x
    Yamamoto, Yusuke ; Banas, Agnieszka ; Murata, Shigenori ; Ishikawa, Madoka ; Lim, Chun R. ; Teratani, Takumi ; Hatada, Izuho ; Matsubara, Kenichi ; Kato, Takashi ; Ochiya, Takahiro. / A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells. In: FEBS Journal. 2008 ; Vol. 275, No. 6. pp. 1260-1273.
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