A concept of image sensor for enzymatic activity

Takaaki Baba, Norikazu Nishino

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This presentation describes an approach towards an enzyme chip, which measures proteolytic activities by the combination of an array of peptide substrates on a solid surface and a high performance image sensor. A number of peptides, which cover the specificities of various proteases in blood, are designed and prepared by chemical synthesis. They are arrayed on a solid surface with micro-channels which drive the test sample solution to the reaction spots. At the linkage between each peptide and the solid surface a fluorescence label is inserted. The reaction of proteases leads to changes in fluorescence intensity at the spots due to their different substrate specificities. The increase in fluorescence intensity is recorded and processed by the image sensor to measure the reaction velocity at each spot. The patterns made of a number of spots are examined and analyzed to detect special physiological aspects of diseases. The enzyme chip is aiming to contribute to the personal healthcare in future.

Original languageEnglish
Title of host publicationDigest of Technical Papers - IEEE International Solid-State Circuits Conference
EditorsL.C. Fujino, M. Amiri, A. Grabel, D. Jaeger, K.C. Smith
Pages683-686
Number of pages4
Volume47
Publication statusPublished - 2003
EventDigest of Technical Papers - IEEE International Solid-State Circuits Conference: Visuals Supplement - San Francisco, CA., United States
Duration: 2003 Feb 152003 Feb 19

Other

OtherDigest of Technical Papers - IEEE International Solid-State Circuits Conference: Visuals Supplement
CountryUnited States
CitySan Francisco, CA.
Period03/2/1503/2/19

Fingerprint

Image sensors
Peptides
Fluorescence
Enzymes
Substrates
Labels
Blood
Peptide Hydrolases

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Hardware and Architecture

Cite this

Baba, T., & Nishino, N. (2003). A concept of image sensor for enzymatic activity. In L. C. Fujino, M. Amiri, A. Grabel, D. Jaeger, & K. C. Smith (Eds.), Digest of Technical Papers - IEEE International Solid-State Circuits Conference (Vol. 47, pp. 683-686)

A concept of image sensor for enzymatic activity. / Baba, Takaaki; Nishino, Norikazu.

Digest of Technical Papers - IEEE International Solid-State Circuits Conference. ed. / L.C. Fujino; M. Amiri; A. Grabel; D. Jaeger; K.C. Smith. Vol. 47 2003. p. 683-686.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Baba, T & Nishino, N 2003, A concept of image sensor for enzymatic activity. in LC Fujino, M Amiri, A Grabel, D Jaeger & KC Smith (eds), Digest of Technical Papers - IEEE International Solid-State Circuits Conference. vol. 47, pp. 683-686, Digest of Technical Papers - IEEE International Solid-State Circuits Conference: Visuals Supplement, San Francisco, CA., United States, 03/2/15.
Baba T, Nishino N. A concept of image sensor for enzymatic activity. In Fujino LC, Amiri M, Grabel A, Jaeger D, Smith KC, editors, Digest of Technical Papers - IEEE International Solid-State Circuits Conference. Vol. 47. 2003. p. 683-686
Baba, Takaaki ; Nishino, Norikazu. / A concept of image sensor for enzymatic activity. Digest of Technical Papers - IEEE International Solid-State Circuits Conference. editor / L.C. Fujino ; M. Amiri ; A. Grabel ; D. Jaeger ; K.C. Smith. Vol. 47 2003. pp. 683-686
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