A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

Kento Yamagishi, Kazuaki Sawaki, Atsushi Murata, Shinji Takeoka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.

Original languageEnglish
Pages (from-to)7879-7882
Number of pages4
JournalChemical Communications
Volume51
Issue number37
DOIs
Publication statusPublished - 2015 May 7

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Azides
Biomolecules
Cell membranes
Mannose
Fluorescent Dyes
Fluorescence
Imaging techniques

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

Cite this

A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules. / Yamagishi, Kento; Sawaki, Kazuaki; Murata, Atsushi; Takeoka, Shinji.

In: Chemical Communications, Vol. 51, No. 37, 07.05.2015, p. 7879-7882.

Research output: Contribution to journalArticle

Yamagishi, Kento ; Sawaki, Kazuaki ; Murata, Atsushi ; Takeoka, Shinji. / A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules. In: Chemical Communications. 2015 ; Vol. 51, No. 37. pp. 7879-7882.
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