A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions

Yukako Hihara, Masayuki Muramatsu, Kinu Nakamura, Kintake Sonoike

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Pirin is a recently identified protein in eukaryotes as a transcription cofactor or as an apoptosis-related protein. Although Pirin is highly conserved from bacteria to human, there have been no reports on prokaryotic Pirin orthologs. We show here that pirA (sll1773) encoding an ortholog of Pirin together with an adjacent gene, pirB (ssl3389), was upregulated under high salinity and some other stress conditions in a cyanobacterium Synechocystis sp. PCC 6803. Induction of the pirAB genes was not related to cell death and disruption of pirA did not affect the gene expression profile. Expression of the pirAB genes was negatively regulated by a LysR family transcriptional regulator encoded by pirR (slr1871) located immediately upstream of pirAB in the divergent direction. DNA microarray analysis indicated that PirR repressed expression of closely located ORFs, slr1870 and mutS (sll1772), in addition to pirAB and pirR itself.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalFEBS Letters
Volume574
Issue number1-3
DOIs
Publication statusPublished - 2004 Sep 10
Externally publishedYes

Fingerprint

Gene encoding
Genes
Synechocystis
Salinity
Cyanobacteria
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Eukaryota
Transcriptome
Open Reading Frames
Proteins
Cell Death
Cell death
Transcription
Microarrays
Apoptosis
Bacteria
Gene Expression
Gene expression
DNA

Keywords

  • DIG, digoxigenin
  • DNA microarray
  • IPTG, isopropyl-β-D(-) -thiogalactopyranoside
  • LysR family
  • PCR, polymerase chain reaction
  • Pirin
  • Salt stress
  • Synechocystis sp. PCC 6803
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions. / Hihara, Yukako; Muramatsu, Masayuki; Nakamura, Kinu; Sonoike, Kintake.

In: FEBS Letters, Vol. 574, No. 1-3, 10.09.2004, p. 101-105.

Research output: Contribution to journalArticle

Hihara, Yukako ; Muramatsu, Masayuki ; Nakamura, Kinu ; Sonoike, Kintake. / A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions. In: FEBS Letters. 2004 ; Vol. 574, No. 1-3. pp. 101-105.
@article{57803c4217944e4dae394934728c8151,
title = "A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions",
abstract = "Pirin is a recently identified protein in eukaryotes as a transcription cofactor or as an apoptosis-related protein. Although Pirin is highly conserved from bacteria to human, there have been no reports on prokaryotic Pirin orthologs. We show here that pirA (sll1773) encoding an ortholog of Pirin together with an adjacent gene, pirB (ssl3389), was upregulated under high salinity and some other stress conditions in a cyanobacterium Synechocystis sp. PCC 6803. Induction of the pirAB genes was not related to cell death and disruption of pirA did not affect the gene expression profile. Expression of the pirAB genes was negatively regulated by a LysR family transcriptional regulator encoded by pirR (slr1871) located immediately upstream of pirAB in the divergent direction. DNA microarray analysis indicated that PirR repressed expression of closely located ORFs, slr1870 and mutS (sll1772), in addition to pirAB and pirR itself.",
keywords = "DIG, digoxigenin, DNA microarray, IPTG, isopropyl-β-D(-) -thiogalactopyranoside, LysR family, PCR, polymerase chain reaction, Pirin, Salt stress, Synechocystis sp. PCC 6803, Transcriptional regulation",
author = "Yukako Hihara and Masayuki Muramatsu and Kinu Nakamura and Kintake Sonoike",
year = "2004",
month = "9",
day = "10",
doi = "10.1016/j.febslet.2004.06.102",
language = "English",
volume = "574",
pages = "101--105",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions

AU - Hihara, Yukako

AU - Muramatsu, Masayuki

AU - Nakamura, Kinu

AU - Sonoike, Kintake

PY - 2004/9/10

Y1 - 2004/9/10

N2 - Pirin is a recently identified protein in eukaryotes as a transcription cofactor or as an apoptosis-related protein. Although Pirin is highly conserved from bacteria to human, there have been no reports on prokaryotic Pirin orthologs. We show here that pirA (sll1773) encoding an ortholog of Pirin together with an adjacent gene, pirB (ssl3389), was upregulated under high salinity and some other stress conditions in a cyanobacterium Synechocystis sp. PCC 6803. Induction of the pirAB genes was not related to cell death and disruption of pirA did not affect the gene expression profile. Expression of the pirAB genes was negatively regulated by a LysR family transcriptional regulator encoded by pirR (slr1871) located immediately upstream of pirAB in the divergent direction. DNA microarray analysis indicated that PirR repressed expression of closely located ORFs, slr1870 and mutS (sll1772), in addition to pirAB and pirR itself.

AB - Pirin is a recently identified protein in eukaryotes as a transcription cofactor or as an apoptosis-related protein. Although Pirin is highly conserved from bacteria to human, there have been no reports on prokaryotic Pirin orthologs. We show here that pirA (sll1773) encoding an ortholog of Pirin together with an adjacent gene, pirB (ssl3389), was upregulated under high salinity and some other stress conditions in a cyanobacterium Synechocystis sp. PCC 6803. Induction of the pirAB genes was not related to cell death and disruption of pirA did not affect the gene expression profile. Expression of the pirAB genes was negatively regulated by a LysR family transcriptional regulator encoded by pirR (slr1871) located immediately upstream of pirAB in the divergent direction. DNA microarray analysis indicated that PirR repressed expression of closely located ORFs, slr1870 and mutS (sll1772), in addition to pirAB and pirR itself.

KW - DIG, digoxigenin

KW - DNA microarray

KW - IPTG, isopropyl-β-D(-) -thiogalactopyranoside

KW - LysR family

KW - PCR, polymerase chain reaction

KW - Pirin

KW - Salt stress

KW - Synechocystis sp. PCC 6803

KW - Transcriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=4444354999&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444354999&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2004.06.102

DO - 10.1016/j.febslet.2004.06.102

M3 - Article

C2 - 15358547

AN - SCOPUS:4444354999

VL - 574

SP - 101

EP - 105

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-3

ER -