A cyclic AMP-regulated negative feedforward system for neuritogenesis revealed in a neuroblastoma × glioma hybrid cell line

T. Tojima, E. Ito

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We examined the role of second messengers during the neuritogenesis that accompanies neuronal differentiation in a neuroblastoma × glioma hybrid cell line (NG108-15). NG108-15 cells extended neurites after treatment with dibutyryl cyclic AMP. This dibutyryl cyclic AMP treatment evoked the synthesis of voltage-dependent Ca2+ channel proteins in the cells. The number of neurites was decreased by Ca2+ influx under condition of high K+. Interestingly, the increase of neurites stimulated by dibutyryl cyclic AMP and the decrease of neurites caused by high K+ were both reversible. This is the first study to demonstrate that cyclic AMP regulates a negative feedforward system for neuritogenesis, which links with Ca2+ signaling. Such a dual role of cyclic AMP may play an important part in precise neurite targeting.

Original languageEnglish
Pages (from-to)583-591
Number of pages9
Issue number2
Publication statusPublished - 2001 May 10



  • Ca
  • NG108-15
  • Neurite
  • Neuronal differentiation
  • Nifedipine
  • Voltage-dependent Ca channel

ASJC Scopus subject areas

  • Neuroscience(all)

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