A cytotoxic ribonuclease targeting specific transfer RNA anticodons

T. Ogawa, K. Tomita, T. Ueda, K. Watanabe, T. Uozumi, H. Masaki

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

The carboxyl-terminal domain of colicin E5 was shown to inhibit protein synthesis of Escherichia coli. Its target, as revealed through in vivo and in vitro experiments, was not ribosomes as in the case of E3, but the transfer RNAs (tRNAs) for Tyr, His, Ash, and Asp, which contain a modified base, queuine, at the wobble position of each anticodon. The E5 carboxyl-terminal domain hydrolyzed these tRNAs just on the 3' side of this nucleotide. Tight correlation was observed between the toxicity of E5 and the cleavage of intracellular tRNAs of this group, implying that these tRNAs are the primary targets of colicin E5.

Original languageEnglish
Pages (from-to)2097-2100
Number of pages4
JournalScience
Volume283
Issue number5410
DOIs
Publication statusPublished - 1999 Mar 26
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Ogawa, T., Tomita, K., Ueda, T., Watanabe, K., Uozumi, T., & Masaki, H. (1999). A cytotoxic ribonuclease targeting specific transfer RNA anticodons. Science, 283(5410), 2097-2100. https://doi.org/10.1126/science.283.5410.2097