A cytotoxic ribonuclease targeting specific transfer RNA anticodons

T. Ogawa, K. Tomita, Takuya Ueda, K. Watanabe, T. Uozumi, H. Masaki

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The carboxyl-terminal domain of colicin E5 was shown to inhibit protein synthesis of Escherichia coli. Its target, as revealed through in vivo and in vitro experiments, was not ribosomes as in the case of E3, but the transfer RNAs (tRNAs) for Tyr, His, Ash, and Asp, which contain a modified base, queuine, at the wobble position of each anticodon. The E5 carboxyl-terminal domain hydrolyzed these tRNAs just on the 3' side of this nucleotide. Tight correlation was observed between the toxicity of E5 and the cleavage of intracellular tRNAs of this group, implying that these tRNAs are the primary targets of colicin E5.

Original languageEnglish
Pages (from-to)2097-2100
Number of pages4
JournalScience
Volume283
Issue number5410
DOIs
Publication statusPublished - 1999 Mar 26
Externally publishedYes

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Anticodon
Ribonucleases
Transfer RNA
Colicins
RNA, Transfer, His
RNA, Transfer, Tyr
Escherichia coli Proteins
Viperidae
Ribosomes
Nucleotides

ASJC Scopus subject areas

  • General

Cite this

Ogawa, T., Tomita, K., Ueda, T., Watanabe, K., Uozumi, T., & Masaki, H. (1999). A cytotoxic ribonuclease targeting specific transfer RNA anticodons. Science, 283(5410), 2097-2100. https://doi.org/10.1126/science.283.5410.2097

A cytotoxic ribonuclease targeting specific transfer RNA anticodons. / Ogawa, T.; Tomita, K.; Ueda, Takuya; Watanabe, K.; Uozumi, T.; Masaki, H.

In: Science, Vol. 283, No. 5410, 26.03.1999, p. 2097-2100.

Research output: Contribution to journalArticle

Ogawa, T, Tomita, K, Ueda, T, Watanabe, K, Uozumi, T & Masaki, H 1999, 'A cytotoxic ribonuclease targeting specific transfer RNA anticodons', Science, vol. 283, no. 5410, pp. 2097-2100. https://doi.org/10.1126/science.283.5410.2097
Ogawa T, Tomita K, Ueda T, Watanabe K, Uozumi T, Masaki H. A cytotoxic ribonuclease targeting specific transfer RNA anticodons. Science. 1999 Mar 26;283(5410):2097-2100. https://doi.org/10.1126/science.283.5410.2097
Ogawa, T. ; Tomita, K. ; Ueda, Takuya ; Watanabe, K. ; Uozumi, T. ; Masaki, H. / A cytotoxic ribonuclease targeting specific transfer RNA anticodons. In: Science. 1999 ; Vol. 283, No. 5410. pp. 2097-2100.
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