A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae

Nao Nishida; Misa Noguchi; Kouichi Kuroda; Mitsuyoshi Ueda

doi:10.1080/09168451.2014.878224

A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae

Nao Nishida, Misa Noguchi, Kouichi Kuroda, Mitsuyoshi Ueda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We have engineered a system that holds potential for use as a safety switch in genetically modified yeasts. Human apoptotic factor BAX (no homolog in yeast), under the control of the FBP1 (gluconeogenesis enzyme) promoter, was conditionally expressed to induce yeast cell apoptosis after glucose depletion. Such systems might prove useful for the safe use of genetically modified organisms.

Original language	English
Pages (from-to)	358-362
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	78
Issue number	2
DOIs	https://doi.org/10.1080/09168451.2014.878224
Publication status	Published - 2014
Externally published	Yes

Keywords

Apoptosis
Bax
Genetically modified organism (GMO)
Gluconeogenesis
Saccharomyces cerevisiae

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2014.878224

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title = "A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae",

abstract = "We have engineered a system that holds potential for use as a safety switch in genetically modified yeasts. Human apoptotic factor BAX (no homolog in yeast), under the control of the FBP1 (gluconeogenesis enzyme) promoter, was conditionally expressed to induce yeast cell apoptosis after glucose depletion. Such systems might prove useful for the safe use of genetically modified organisms.",

keywords = "Apoptosis, Bax, Genetically modified organism (GMO), Gluconeogenesis, Saccharomyces cerevisiae",

author = "Nao Nishida and Misa Noguchi and Kouichi Kuroda and Mitsuyoshi Ueda",

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T1 - A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae

AU - Nishida, Nao

AU - Noguchi, Misa

AU - Kuroda, Kouichi

AU - Ueda, Mitsuyoshi

N1 - Funding Information: This work was supported by the commission for Development of Artificial Genes Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade, and Industry (METI), Japan. Publisher Copyright: © 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

PY - 2014

Y1 - 2014

N2 - We have engineered a system that holds potential for use as a safety switch in genetically modified yeasts. Human apoptotic factor BAX (no homolog in yeast), under the control of the FBP1 (gluconeogenesis enzyme) promoter, was conditionally expressed to induce yeast cell apoptosis after glucose depletion. Such systems might prove useful for the safe use of genetically modified organisms.

AB - We have engineered a system that holds potential for use as a safety switch in genetically modified yeasts. Human apoptotic factor BAX (no homolog in yeast), under the control of the FBP1 (gluconeogenesis enzyme) promoter, was conditionally expressed to induce yeast cell apoptosis after glucose depletion. Such systems might prove useful for the safe use of genetically modified organisms.

KW - Apoptosis

KW - Bax

KW - Genetically modified organism (GMO)

KW - Gluconeogenesis

KW - Saccharomyces cerevisiae

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JF - Bioscience, Biotechnology and Biochemistry

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ER -

A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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