A DIGE proteomic analysis for high-intensity exercise-trained rat skeletal muscle

Wataru Yamaguchi, Eri Takai, Mitsuru Higuchi, Izumi Tabata

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26 Citations (Scopus)

Abstract

Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. In this study, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after high-intensity exercise training (HIT). For 5 days, rats performed HIT, which consisted of 14 20-s swimming exercise bouts carrying a weight (14 of the body weight), and 10-s pause between bouts. The 2D-DIGE analysis was conducted on epitrochlearis muscles excised 18 h after the final training exercise. Proteomic profiling revealed that out of 800 detected and matched spots, 13 proteins exhibited changed expression by HIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Furthermore, using western immunoblot analyses, significantly changed expressions of NDUFS1 and parvalbumin (PV) were validated in relation to HIT. In conclusion, the proteomic 2D-DIGE analysis following HIT-identified expressions of NDUFS1 and PV, previously unknown to have functions related to exercise-training adaptations.

Original languageEnglish
Pages (from-to)327-333
Number of pages7
JournalJournal of Biochemistry
Volume148
Issue number3
DOIs
Publication statusPublished - 2010 Sep

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Keywords

  • 2D-DIGE
  • exercise training
  • NDUFS1
  • parvalbumin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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