Abstract
Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. In this study, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after high-intensity exercise training (HIT). For 5 days, rats performed HIT, which consisted of 14 20-s swimming exercise bouts carrying a weight (14 of the body weight), and 10-s pause between bouts. The 2D-DIGE analysis was conducted on epitrochlearis muscles excised 18 h after the final training exercise. Proteomic profiling revealed that out of 800 detected and matched spots, 13 proteins exhibited changed expression by HIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Furthermore, using western immunoblot analyses, significantly changed expressions of NDUFS1 and parvalbumin (PV) were validated in relation to HIT. In conclusion, the proteomic 2D-DIGE analysis following HIT-identified expressions of NDUFS1 and PV, previously unknown to have functions related to exercise-training adaptations.
| Original language | English |
|---|---|
| Pages (from-to) | 327-333 |
| Number of pages | 7 |
| Journal | Journal of Biochemistry |
| Volume | 148 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 Sep |
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Keywords
- 2D-DIGE
- exercise training
- NDUFS1
- parvalbumin
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
A DIGE proteomic analysis for high-intensity exercise-trained rat skeletal muscle. / Yamaguchi, Wataru; Takai, Eri; Higuchi, Mitsuru; Tabata, Izumi.
In: Journal of Biochemistry, Vol. 148, No. 3, 09.2010, p. 327-333.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A DIGE proteomic analysis for high-intensity exercise-trained rat skeletal muscle
AU - Yamaguchi, Wataru
AU - Takai, Eri
AU - Higuchi, Mitsuru
AU - Tabata, Izumi
PY - 2010/9
Y1 - 2010/9
N2 - Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. In this study, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after high-intensity exercise training (HIT). For 5 days, rats performed HIT, which consisted of 14 20-s swimming exercise bouts carrying a weight (14 of the body weight), and 10-s pause between bouts. The 2D-DIGE analysis was conducted on epitrochlearis muscles excised 18 h after the final training exercise. Proteomic profiling revealed that out of 800 detected and matched spots, 13 proteins exhibited changed expression by HIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Furthermore, using western immunoblot analyses, significantly changed expressions of NDUFS1 and parvalbumin (PV) were validated in relation to HIT. In conclusion, the proteomic 2D-DIGE analysis following HIT-identified expressions of NDUFS1 and PV, previously unknown to have functions related to exercise-training adaptations.
AB - Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. In this study, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after high-intensity exercise training (HIT). For 5 days, rats performed HIT, which consisted of 14 20-s swimming exercise bouts carrying a weight (14 of the body weight), and 10-s pause between bouts. The 2D-DIGE analysis was conducted on epitrochlearis muscles excised 18 h after the final training exercise. Proteomic profiling revealed that out of 800 detected and matched spots, 13 proteins exhibited changed expression by HIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Furthermore, using western immunoblot analyses, significantly changed expressions of NDUFS1 and parvalbumin (PV) were validated in relation to HIT. In conclusion, the proteomic 2D-DIGE analysis following HIT-identified expressions of NDUFS1 and PV, previously unknown to have functions related to exercise-training adaptations.
KW - 2D-DIGE
KW - exercise training
KW - NDUFS1
KW - parvalbumin
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UR - http://www.scopus.com/inward/citedby.url?scp=77956441071&partnerID=8YFLogxK
U2 - 10.1093/jb/mvq073
DO - 10.1093/jb/mvq073
M3 - Article
C2 - 20634418
AN - SCOPUS:77956441071
VL - 148
SP - 327
EP - 333
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 3
ER -