A DIGE proteomic analysis for low-intensity exercise-trained rat skeletal muscle

Wataru Yamaguchi, Eri Takai, Mitsuru Higuchi, Izumi Tabata

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. Purpose: Therefore, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after low-intensity exercise training (LIT). Methods: For five days, rats performed LIT, which consisted of two 3-h swimming exercise with45-m rest between the exercise bouts. 2D-DIGE analysis was conducted on epitrochlearis muscles excised eighteen hours after the final training exercise. Results: Proteomic profiling revealed that, out of 681 detected and matched spots, 22 proteins exhibited changed expression by LIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Conclusion: The proteomic 2D-DIGE analysis following LIT identified expressions of skeletal muscle proteins, includingATPsyna, UQCRC1, dihydrolipoamide dehydrogenase, dihydrolipoamide acetyltransferase, that were not previously reported to change their expressions after exercise-training.

Original languageEnglish
Pages (from-to)511-518
Number of pages8
JournalJapanese Journal of Physical Fitness and Sports Medicine
Volume60
Issue number5
Publication statusPublished - 2011

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Proteomics
Skeletal Muscle
Exercise
Two-Dimensional Difference Gel Electrophoresis
Dihydrolipoyllysine-Residue Acetyltransferase
Dihydrolipoamide Dehydrogenase
Muscle Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Proteins
Muscles

Keywords

  • Epitrochlearis
  • Low-intensity exercise training
  • Proteomics
  • Swimming

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

A DIGE proteomic analysis for low-intensity exercise-trained rat skeletal muscle. / Yamaguchi, Wataru; Takai, Eri; Higuchi, Mitsuru; Tabata, Izumi.

In: Japanese Journal of Physical Fitness and Sports Medicine, Vol. 60, No. 5, 2011, p. 511-518.

Research output: Contribution to journalArticle

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