A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes

Fernando López-Redondo, Junko Kurokawa, Fumimasa Nomura, Tomoyuki Kaneko, Tomoyo Hamada, Tetsushi Furukawa, Kenji Yasuda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes, and analyzed action potential parameters by plotting their frequency distributions. In the both cell lines, the distribution analysis revealed that histograms of maximum upstroke velocity showed two subpopulations with similar intersection values. Sub-populations with faster maximum upstroke velocity showed significant prolongation of action potential durations by application of E-4031, whereas others did not, which may be partly due to shallower maximum diastolic potentials. We described electrophysiological and pharmacological properties of stem-cell-derived cardiomyocytes in the respective sub-populations, which provides a way to characterize diverse electrical properties of stem-cell-derived cardiomyocytes systematically.

Original languageEnglish
JournalJournal of Pharmacological Sciences
DOIs
Publication statusAccepted/In press - 2015 Dec 11
Externally publishedYes

Keywords

  • Cardiomyocyte
  • IPS cells
  • Patch-clamp

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

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