TY - JOUR
T1 - A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
AU - López-Redondo, Fernando
AU - Kurokawa, Junko
AU - Nomura, Fumimasa
AU - Kaneko, Tomoyuki
AU - Hamada, Tomoyo
AU - Furukawa, Tetsushi
AU - Yasuda, Kenji
N1 - Funding Information:
This research was supported by the Japan New Energy and Industrial Technology Development Organization (NEDO) ( P08030 ). The authors are grateful to Ms. Reiko Osumi, Ms. Reiko Kimura, Ms. Akiko Hagiya, Ms. Tamae Takato and Ms. Naoko Ishii for technical assistance.
Publisher Copyright:
© 2016 The Authors
PY - 2016/6/1
Y1 - 2016/6/1
N2 - We investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes, and analyzed action potential parameters by plotting their frequency distributions. In the both cell lines, the distribution analysis revealed that histograms of maximum upstroke velocity showed two subpopulations with similar intersection values. Sub-populations with faster maximum upstroke velocity showed significant prolongation of action potential durations by application of E-4031, whereas others did not, which may be partly due to shallower maximum diastolic potentials. We described electrophysiological and pharmacological properties of stem-cell-derived cardiomyocytes in the respective sub-populations, which provides a way to characterize diverse electrical properties of stem-cell-derived cardiomyocytes systematically.
AB - We investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes, and analyzed action potential parameters by plotting their frequency distributions. In the both cell lines, the distribution analysis revealed that histograms of maximum upstroke velocity showed two subpopulations with similar intersection values. Sub-populations with faster maximum upstroke velocity showed significant prolongation of action potential durations by application of E-4031, whereas others did not, which may be partly due to shallower maximum diastolic potentials. We described electrophysiological and pharmacological properties of stem-cell-derived cardiomyocytes in the respective sub-populations, which provides a way to characterize diverse electrical properties of stem-cell-derived cardiomyocytes systematically.
KW - Cardiomyocyte
KW - Patch-clamp
KW - iPS cells
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U2 - 10.1016/j.jphs.2016.04.015
DO - 10.1016/j.jphs.2016.04.015
M3 - Article
C2 - 27179939
AN - SCOPUS:84969913502
SN - 1347-8613
VL - 131
SP - 141
EP - 145
JO - Journal of Pharmacological Sciences
JF - Journal of Pharmacological Sciences
IS - 2
ER -