Abstract
The excitatory amino acid, glutamate plays a crucial role in the pathogenesis of brain damage caused by anoxia and/or hypoglycemia. Although vasopressin (VP) also acts as an excitatory transmitter in the CNS, little is known about its effect on hypoxic and/or ischemic brain damage. In this study, we investigated the effect of arginine vasopressin (AVP) on hypoxia/hypoglycemia-induced impairment of dopamine release from striatal slices. Striatal slices were incubated in hypoxia-/hypoglycemia-inducing medium with or without AVP (0.01-1.0 μM) for 20 min. After 1-3 h of washout in normal medium, high K+-evoked dopamine release from the slices were examined. Hypoxia/hypoglycemia-induced decrease of striatal dopamine release was reversed by the removal of Ca2+ in the medium, but not by VP1- or VP2-receptor antagonist. In contrast, AVP potentiated the hypoxia/hypoglycemia-induced decrease of dopamine release in the striatum. This AVP-induced deterioration of the striatal response was antagonized by VP2 receptor antagonist, but not by VP1 receptor antagonist. The present results suggest that AVP may play a facilitatory role in hypoxia/hypoglycemia-induced dopamine release deficit mediated through the activation of VP2 receptor.
| Original language | English |
|---|---|
| Pages (from-to) | 91-96 |
| Number of pages | 6 |
| Journal | Brain Research |
| Volume | 633 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1994 Jan 7 |
| Externally published | Yes |
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Keywords
- Brain slice
- Dopamine release
- Hypoxia/hypoglycemia
- Ischemia
- Striatum
- Vasopressin
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
A facilitatory role of vasopressin in hypoxia/hypoglycemia-induced impairment of dopamine release from rat striatal slices. / Ochi, Mayumi; Koizumi, Schuichi; Shibata, Shigenobu; Watanabe, Shigenori.
In: Brain Research, Vol. 633, No. 1-2, 07.01.1994, p. 91-96.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A facilitatory role of vasopressin in hypoxia/hypoglycemia-induced impairment of dopamine release from rat striatal slices
AU - Ochi, Mayumi
AU - Koizumi, Schuichi
AU - Shibata, Shigenobu
AU - Watanabe, Shigenori
PY - 1994/1/7
Y1 - 1994/1/7
N2 - The excitatory amino acid, glutamate plays a crucial role in the pathogenesis of brain damage caused by anoxia and/or hypoglycemia. Although vasopressin (VP) also acts as an excitatory transmitter in the CNS, little is known about its effect on hypoxic and/or ischemic brain damage. In this study, we investigated the effect of arginine vasopressin (AVP) on hypoxia/hypoglycemia-induced impairment of dopamine release from striatal slices. Striatal slices were incubated in hypoxia-/hypoglycemia-inducing medium with or without AVP (0.01-1.0 μM) for 20 min. After 1-3 h of washout in normal medium, high K+-evoked dopamine release from the slices were examined. Hypoxia/hypoglycemia-induced decrease of striatal dopamine release was reversed by the removal of Ca2+ in the medium, but not by VP1- or VP2-receptor antagonist. In contrast, AVP potentiated the hypoxia/hypoglycemia-induced decrease of dopamine release in the striatum. This AVP-induced deterioration of the striatal response was antagonized by VP2 receptor antagonist, but not by VP1 receptor antagonist. The present results suggest that AVP may play a facilitatory role in hypoxia/hypoglycemia-induced dopamine release deficit mediated through the activation of VP2 receptor.
AB - The excitatory amino acid, glutamate plays a crucial role in the pathogenesis of brain damage caused by anoxia and/or hypoglycemia. Although vasopressin (VP) also acts as an excitatory transmitter in the CNS, little is known about its effect on hypoxic and/or ischemic brain damage. In this study, we investigated the effect of arginine vasopressin (AVP) on hypoxia/hypoglycemia-induced impairment of dopamine release from striatal slices. Striatal slices were incubated in hypoxia-/hypoglycemia-inducing medium with or without AVP (0.01-1.0 μM) for 20 min. After 1-3 h of washout in normal medium, high K+-evoked dopamine release from the slices were examined. Hypoxia/hypoglycemia-induced decrease of striatal dopamine release was reversed by the removal of Ca2+ in the medium, but not by VP1- or VP2-receptor antagonist. In contrast, AVP potentiated the hypoxia/hypoglycemia-induced decrease of dopamine release in the striatum. This AVP-induced deterioration of the striatal response was antagonized by VP2 receptor antagonist, but not by VP1 receptor antagonist. The present results suggest that AVP may play a facilitatory role in hypoxia/hypoglycemia-induced dopamine release deficit mediated through the activation of VP2 receptor.
KW - Brain slice
KW - Dopamine release
KW - Hypoxia/hypoglycemia
KW - Ischemia
KW - Striatum
KW - Vasopressin
UR - http://www.scopus.com/inward/record.url?scp=0028069327&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028069327&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(94)91526-1
DO - 10.1016/0006-8993(94)91526-1
M3 - Article
VL - 633
SP - 91
EP - 96
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -