A genetic study of inositol trisphosphate involvement in phototransduction using Drosophila mutants

Hiroko Inoue, Tohru Yoshioka, Yoshiki Hotta

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Phosphatidylinositol 4,5-bisphosphate phosphodiesterase activity was found to be almost absent in the compound eyes of Drosophila visual mutant, norpA (no receptor potentials A). We compared the enzyme activities among independently isolated norpA alleles, each having a different degree of the vision defect. A close correlation was found between the size of receptor potentials (electroretinogram), phototactic behavior and the enzyme activity. The correlation exists not only among alleles, but also in a single, temperature-dependent allele under different temperature; the enzyme activity of flies kept at 18°C (phototactic) was about five times higher than that of the ones kept at 28°C (blind). These results suggest that hydrolysis of phosphatidylinositol 4,5-bisphosphate is involved in phototransduction process in Drosophila eyes.

Original languageEnglish
Pages (from-to)513-519
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume132
Issue number2
DOIs
Publication statusPublished - 1985 Oct 30
Externally publishedYes

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Light Signal Transduction
Enzyme activity
Inositol
Drosophila
Alleles
Phosphatidylinositols
Enzymes
Type 5 Cyclic Nucleotide Phosphodiesterases
Temperature
Phosphoric Diester Hydrolases
Diptera
Hydrolysis
Defects

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A genetic study of inositol trisphosphate involvement in phototransduction using Drosophila mutants. / Inoue, Hiroko; Yoshioka, Tohru; Hotta, Yoshiki.

In: Biochemical and Biophysical Research Communications, Vol. 132, No. 2, 30.10.1985, p. 513-519.

Research output: Contribution to journalArticle

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