A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras

Fumihiko Maekawa, Miyano Sakurai, Yuki Yamashita, Kohichi Tanaka, Shogo Haraguchi, Kazutoshi Yamamoto, Kazuyoshi Tsutsui, Hidefumi Yoshioka, Shizuko Murakami, Ryo Tadano, Tatsuhiko Goto, Jun Ichi Shiraishi, Kohei Tomonari, Takao Oka, Ken Ohara, Teruo Maeda, Takashi Bungo, Masaoki Tsudzuki, Hiroko Ohki-Hamazaki

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Sexual differentiation leads to structural and behavioural differences between males and females. Here we investigate the intrinsic sex identity of the brain by constructing chicken chimeras in which the brain primordium is switched between male and female identities before gonadal development. We find that the female chimeras with male brains display delayed sexual maturation and irregular oviposition cycles, although their behaviour, plasma concentrations of sex steroids and luteinizing hormone levels are normal. The male chimeras with female brains show phenotypes similar to typical cocks. In the perinatal period, oestrogen concentrations in the genetically male brain are higher than those in the genetically female brain. Our study demonstrates that male brain cells retain male sex identity and do not differentiate into female cells to drive the normal oestrous cycle, even when situated in the female hormonal milieu. This is clear evidence for a sex-specific feature that develops independent of gonadal steroids.

    Original languageEnglish
    Article number1372
    JournalNature Communications
    Volume4
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    chickens
    brain
    Chickens
    Brain
    cycles
    steroids
    cocks
    estrogens
    Sexual Maturation
    Oviposition
    Sex Differentiation
    phenotype
    hormones
    Gonadal Steroid Hormones
    Luteinizing Hormone
    cells
    Estrogens
    Steroids
    Phenotype
    Plasmas

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Chemistry(all)
    • Physics and Astronomy(all)

    Cite this

    Maekawa, F., Sakurai, M., Yamashita, Y., Tanaka, K., Haraguchi, S., Yamamoto, K., ... Ohki-Hamazaki, H. (2013). A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras. Nature Communications, 4, [1372]. https://doi.org/10.1038/ncomms2372

    A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras. / Maekawa, Fumihiko; Sakurai, Miyano; Yamashita, Yuki; Tanaka, Kohichi; Haraguchi, Shogo; Yamamoto, Kazutoshi; Tsutsui, Kazuyoshi; Yoshioka, Hidefumi; Murakami, Shizuko; Tadano, Ryo; Goto, Tatsuhiko; Shiraishi, Jun Ichi; Tomonari, Kohei; Oka, Takao; Ohara, Ken; Maeda, Teruo; Bungo, Takashi; Tsudzuki, Masaoki; Ohki-Hamazaki, Hiroko.

    In: Nature Communications, Vol. 4, 1372, 2013.

    Research output: Contribution to journalArticle

    Maekawa, F, Sakurai, M, Yamashita, Y, Tanaka, K, Haraguchi, S, Yamamoto, K, Tsutsui, K, Yoshioka, H, Murakami, S, Tadano, R, Goto, T, Shiraishi, JI, Tomonari, K, Oka, T, Ohara, K, Maeda, T, Bungo, T, Tsudzuki, M & Ohki-Hamazaki, H 2013, 'A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras', Nature Communications, vol. 4, 1372. https://doi.org/10.1038/ncomms2372
    Maekawa, Fumihiko ; Sakurai, Miyano ; Yamashita, Yuki ; Tanaka, Kohichi ; Haraguchi, Shogo ; Yamamoto, Kazutoshi ; Tsutsui, Kazuyoshi ; Yoshioka, Hidefumi ; Murakami, Shizuko ; Tadano, Ryo ; Goto, Tatsuhiko ; Shiraishi, Jun Ichi ; Tomonari, Kohei ; Oka, Takao ; Ohara, Ken ; Maeda, Teruo ; Bungo, Takashi ; Tsudzuki, Masaoki ; Ohki-Hamazaki, Hiroko. / A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras. In: Nature Communications. 2013 ; Vol. 4.
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    abstract = "Sexual differentiation leads to structural and behavioural differences between males and females. Here we investigate the intrinsic sex identity of the brain by constructing chicken chimeras in which the brain primordium is switched between male and female identities before gonadal development. We find that the female chimeras with male brains display delayed sexual maturation and irregular oviposition cycles, although their behaviour, plasma concentrations of sex steroids and luteinizing hormone levels are normal. The male chimeras with female brains show phenotypes similar to typical cocks. In the perinatal period, oestrogen concentrations in the genetically male brain are higher than those in the genetically female brain. Our study demonstrates that male brain cells retain male sex identity and do not differentiate into female cells to drive the normal oestrous cycle, even when situated in the female hormonal milieu. This is clear evidence for a sex-specific feature that develops independent of gonadal steroids.",
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