A gonad-stimulating peptide of the crown-of-thorns starfish, Acanthaster planci

Masatoshi Mita, Narumi Ikeda, Shogo Haraguchi, Kazuyoshi Tsutsui, Yoshikatsu Nakano, Masaru Nakamura

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    The crown-of thorns starfish, Acanthaster planci, has been blamed for coral mortality in a large number of coral reef systems in the Indo-Pacific region. Because population outbreaks of A. planci are closely related to reproduction, it is important to examine the mechanism of reproductive control in this starfish. Previously, a relaxin-like gonad-stimulating peptide (RGP) in starfish Asterina pectinifera has been identified as the gonadotropin responsible for final gamete maturation. On the basis of homology research on RGP cDNAs from several species, this study was carried out to identify gonadotropin in A. planci. The cDNA sequence of RGP was determined using a RACE product of mRNA from the radial nerves of A. planci. The coding DNA sequence consisted of 351 base pairs with an open reading frame encoding a peptide of 116 amino acids (aa), including a signal peptide (29 aa), B-chain (19 aa), C-peptide (44 aa), and A-chain (24 aa). The chemical structure of A. planci RGP was exactly the same as that of A. pectinifera RGP. Furthermore, synthetic RGP could induce gamete spawning and oocyte maturation in the ovarian fragments of A. planci. This strongly suggested that the RGP is a gonadotropin in A. planci.

    Original languageEnglish
    JournalInvertebrate Reproduction and Development
    DOIs
    Publication statusAccepted/In press - 2015 Sep 13

    Fingerprint

    Starfish
    Acanthaster planci
    Relaxin
    relaxin
    Gonads
    Crowns
    gonads
    peptides
    Peptides
    Asterina
    Amino Acids
    Gonadotropins
    Asterina pectinifera
    gonadotropins
    amino acids
    Asteroidea
    Germ Cells
    Complementary DNA
    germ cells
    Coral Reefs

    Keywords

    • crown-of-thorns starfish (Acanthaster planci)
    • gonadotropin
    • radial nerves
    • relaxin-like gonad-stimulating peptide

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Developmental Biology

    Cite this

    A gonad-stimulating peptide of the crown-of-thorns starfish, Acanthaster planci. / Mita, Masatoshi; Ikeda, Narumi; Haraguchi, Shogo; Tsutsui, Kazuyoshi; Nakano, Yoshikatsu; Nakamura, Masaru.

    In: Invertebrate Reproduction and Development, 13.09.2015.

    Research output: Contribution to journalArticle

    Mita, Masatoshi ; Ikeda, Narumi ; Haraguchi, Shogo ; Tsutsui, Kazuyoshi ; Nakano, Yoshikatsu ; Nakamura, Masaru. / A gonad-stimulating peptide of the crown-of-thorns starfish, Acanthaster planci. In: Invertebrate Reproduction and Development. 2015.
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