Abstract
The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
| Original language | English |
|---|---|
| Pages (from-to) | 267-271 |
| Number of pages | 5 |
| Journal | Current Pharmaceutical Biotechnology |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 Apr 1 |
| Externally published | Yes |
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Keywords
- Cell-free protein synthesis
- Chaperone
- Disulfide bond formation
- In vitro selection
- In vitro translation
- Unnatural amino acid
ASJC Scopus subject areas
- Pharmaceutical Science
- Biotechnology
Cite this
A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research. / Ohashi, Hiroyuki; Kanamori, Takashi; Shimizu, Yoshihiro; Ueda, Takuya.
In: Current Pharmaceutical Biotechnology, Vol. 11, No. 3, 01.04.2010, p. 267-271.Research output: Contribution to journal › Short survey
}
TY - JOUR
T1 - A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research
AU - Ohashi, Hiroyuki
AU - Kanamori, Takashi
AU - Shimizu, Yoshihiro
AU - Ueda, Takuya
PY - 2010/4/1
Y1 - 2010/4/1
N2 - The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
AB - The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
KW - Cell-free protein synthesis
KW - Chaperone
KW - Disulfide bond formation
KW - In vitro selection
KW - In vitro translation
KW - Unnatural amino acid
UR - http://www.scopus.com/inward/record.url?scp=77950872668&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950872668&partnerID=8YFLogxK
U2 - 10.2174/138920110791111889
DO - 10.2174/138920110791111889
M3 - Short survey
VL - 11
SP - 267
EP - 271
JO - Current Pharmaceutical Biotechnology
JF - Current Pharmaceutical Biotechnology
SN - 1389-2010
IS - 3
ER -