A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

Hiroyuki Ohashi; Takashi Kanamori; Yoshihiro Shimizu; Takuya Ueda

doi:10.2174/138920110791111889

A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

Hiroyuki Ohashi, Takashi Kanamori, Yoshihiro Shimizu, Takuya Ueda^*

^*Corresponding author for this work

Research output: Contribution to journal › Short survey › peer-review

38 Citations (Scopus)

Abstract

The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

Original language	English
Pages (from-to)	267-271
Number of pages	5
Journal	Current Pharmaceutical Biotechnology
Volume	11
Issue number	3
DOIs	https://doi.org/10.2174/138920110791111889
Publication status	Published - 2010 Apr 1
Externally published	Yes

Keywords

Cell-free protein synthesis
Chaperone
Disulfide bond formation
In vitro selection
In vitro translation
Unnatural amino acid

ASJC Scopus subject areas

Biotechnology
Pharmaceutical Science

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10.2174/138920110791111889

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abstract = "The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.",

keywords = "Cell-free protein synthesis, Chaperone, Disulfide bond formation, In vitro selection, In vitro translation, Unnatural amino acid",

author = "Hiroyuki Ohashi and Takashi Kanamori and Yoshihiro Shimizu and Takuya Ueda",

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T1 - A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

AU - Ohashi, Hiroyuki

AU - Kanamori, Takashi

AU - Shimizu, Yoshihiro

AU - Ueda, Takuya

PY - 2010/4/1

Y1 - 2010/4/1

N2 - The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

AB - The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

KW - Cell-free protein synthesis

KW - Chaperone

KW - Disulfide bond formation

KW - In vitro selection

KW - In vitro translation

KW - Unnatural amino acid

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M3 - Short survey

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JO - Current Pharmaceutical Biotechnology

JF - Current Pharmaceutical Biotechnology

SN - 1389-2010

IS - 3

ER -

A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

Abstract

Keywords

ASJC Scopus subject areas

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