A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

Hiroyuki Ohashi; Takashi Kanamori; Yoshihiro Shimizu; Takuya Ueda

doi:10.2174/138920110791111889

A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

Hiroyuki Ohashi, Takashi Kanamori, Yoshihiro Shimizu, Takuya Ueda

Research output: Contribution to journal › Short survey › peer-review

36 Citations (Scopus)

Abstract

The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

Original language	English
Pages (from-to)	267-271
Number of pages	5
Journal	Current Pharmaceutical Biotechnology
Volume	11
Issue number	3
DOIs	https://doi.org/10.2174/138920110791111889
Publication status	Published - 2010 Apr 1
Externally published	Yes

Keywords

Cell-free protein synthesis
Chaperone
Disulfide bond formation
In vitro selection
In vitro translation
Unnatural amino acid

ASJC Scopus subject areas

Biotechnology
Pharmaceutical Science

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AU - Ohashi, Hiroyuki

AU - Kanamori, Takashi

AU - Shimizu, Yoshihiro

AU - Ueda, Takuya

PY - 2010/4/1

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AB - The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

KW - Cell-free protein synthesis

KW - Chaperone

KW - Disulfide bond formation

KW - In vitro selection

KW - In vitro translation

KW - Unnatural amino acid

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