A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

Hiroyuki Ohashi, Takashi Kanamori, Yoshihiro Shimizu, Takuya Ueda

Research output: Contribution to journalShort survey

35 Citations (Scopus)

Abstract

The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

Original languageEnglish
Pages (from-to)267-271
Number of pages5
JournalCurrent Pharmaceutical Biotechnology
Volume11
Issue number3
DOIs
Publication statusPublished - 2010 Apr 1
Externally publishedYes

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Keywords

  • Cell-free protein synthesis
  • Chaperone
  • Disulfide bond formation
  • In vitro selection
  • In vitro translation
  • Unnatural amino acid

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Biotechnology

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