A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

Hiroyuki Ohashi, Takashi Kanamori, Yoshihiro Shimizu, Takuya Ueda

Research output: Contribution to journalShort survey

33 Citations (Scopus)

Abstract

The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

Original languageEnglish
Pages (from-to)267-271
Number of pages5
JournalCurrent Pharmaceutical Biotechnology
Volume11
Issue number3
DOIs
Publication statusPublished - 2010 Apr 1
Externally publishedYes

Fingerprint

Cell-Free System
Research
Proteins
Affinity Chromatography
Peptide Hydrolases
Escherichia coli
Messenger RNA
DNA

Keywords

  • Cell-free protein synthesis
  • Chaperone
  • Disulfide bond formation
  • In vitro selection
  • In vitro translation
  • Unnatural amino acid

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Biotechnology

Cite this

A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research. / Ohashi, Hiroyuki; Kanamori, Takashi; Shimizu, Yoshihiro; Ueda, Takuya.

In: Current Pharmaceutical Biotechnology, Vol. 11, No. 3, 01.04.2010, p. 267-271.

Research output: Contribution to journalShort survey

Ohashi, Hiroyuki ; Kanamori, Takashi ; Shimizu, Yoshihiro ; Ueda, Takuya. / A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research. In: Current Pharmaceutical Biotechnology. 2010 ; Vol. 11, No. 3. pp. 267-271.
@article{91cda062d0d54583b1cf3efe6e9e8e8e,
title = "A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research",
abstract = "The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.",
keywords = "Cell-free protein synthesis, Chaperone, Disulfide bond formation, In vitro selection, In vitro translation, Unnatural amino acid",
author = "Hiroyuki Ohashi and Takashi Kanamori and Yoshihiro Shimizu and Takuya Ueda",
year = "2010",
month = "4",
day = "1",
doi = "10.2174/138920110791111889",
language = "English",
volume = "11",
pages = "267--271",
journal = "Current Pharmaceutical Biotechnology",
issn = "1389-2010",
publisher = "Bentham Science Publishers B.V.",
number = "3",

}

TY - JOUR

T1 - A highly controllable reconstituted cell-free system -a breakthrough in protein synthesis research

AU - Ohashi, Hiroyuki

AU - Kanamori, Takashi

AU - Shimizu, Yoshihiro

AU - Ueda, Takuya

PY - 2010/4/1

Y1 - 2010/4/1

N2 - The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

AB - The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

KW - Cell-free protein synthesis

KW - Chaperone

KW - Disulfide bond formation

KW - In vitro selection

KW - In vitro translation

KW - Unnatural amino acid

UR - http://www.scopus.com/inward/record.url?scp=77950872668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950872668&partnerID=8YFLogxK

U2 - 10.2174/138920110791111889

DO - 10.2174/138920110791111889

M3 - Short survey

VL - 11

SP - 267

EP - 271

JO - Current Pharmaceutical Biotechnology

JF - Current Pharmaceutical Biotechnology

SN - 1389-2010

IS - 3

ER -