A histone H1 variant is required for erythrocyte maturation in medaka

Osamu Matsuoka, Norihisa Shindo, Daisuke Arai, Toru Higashinakagawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Three histone H1 variants were identified in medaka fish and their sequence characteristics were analyzed. This paper reports one of these variants, termed H1-2, because of its possible implication in erythrocyte maturation. The amino acid sequence of H1-2 was phylogenetically similar to that of other replication-dependent histones. The mRNA transcribed from the h1-2 gene, however, possessed a poly(A) tail without a stem-loop structure, indicating that H1-2 may represent a replication-independent (RI) histone. Transcripts from the h1-2 gene were largely localized in erythrocytes, and knock-down of the h1-2 gene with morpholino antisense oligos resulted in failure to develop mature erythrocytes. In the morphants, residual erythrocytes showed severely impaired nuclear compaction. Although not structurally related to Chicken RI histone H5, which is required for erythrocyte maturation, H1-2 may constitute its functional counterpart. Our findings may offer evolutionary insights into the function of H1 variants.

Original languageEnglish
Pages (from-to)887-892
Number of pages6
JournalInternational Journal of Developmental Biology
Volume52
Issue number7
DOIs
Publication statusPublished - 2008

Fingerprint

Oryzias
Histones
Erythrocytes
Genes
Morpholinos
Messenger RNA
Amino Acid Sequence
Chickens
Fishes

Keywords

  • Evolution
  • Medaka
  • Replication-dependent histone
  • Replication-indegendent histone

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

Cite this

A histone H1 variant is required for erythrocyte maturation in medaka. / Matsuoka, Osamu; Shindo, Norihisa; Arai, Daisuke; Higashinakagawa, Toru.

In: International Journal of Developmental Biology, Vol. 52, No. 7, 2008, p. 887-892.

Research output: Contribution to journalArticle

Matsuoka, Osamu ; Shindo, Norihisa ; Arai, Daisuke ; Higashinakagawa, Toru. / A histone H1 variant is required for erythrocyte maturation in medaka. In: International Journal of Developmental Biology. 2008 ; Vol. 52, No. 7. pp. 887-892.
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