A KaiC-associating SasA-RpaA two-component regulatory system as a major circadian timing mediator in cyanobacteria

Naoki Takai, Masato Nakajima, Tokitaka Oyama, Ryotaku Kito, Chieko Sugita, Mamoru Sugita, Takao Kondo, Hideo Iwasaki

    Research output: Contribution to journalArticle

    149 Citations (Scopus)

    Abstract

    KaiA, KaiB, and KaiC clock proteins from cyanobacteria and ATP are sufficient to reconstitute the KaiC phosphorylation rhythm in vitro, whereas almost all gene promoters are under the control of the circadian clock. The mechanism by which the KaiC phosphorylation cycle drives global transcription rhythms is unknown. Here, we report that RpaA, a potential DNA-binding protein that acts as a cognate response regulator of the KaiC-interacting kinase SasA, mediates between KaiC phosphorylation and global transcription rhythms. Circadian transcription was severely attenuated in sasA (Synechococcus adaptive sensor A)- and rpaA (regulator of phycobilisome-associated)-mutant cells, and the phosphotransfer activity from SasA to RpaA changed dramatically depending on the circadian state of a coexisting Kai protein complex in vitro. We propose a model in which the SasA-RpaA two-component system mediates time signals from the enzymatic oscillator to drive genome-wide transcription rhythms in cyanobacteria. Moreover, our results indicate the presence of secondary output pathways from the clock to transcription control, suggesting that multiple pathways ensure a genome-wide circadian system.

    Original languageEnglish
    Pages (from-to)12109-12114
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume103
    Issue number32
    DOIs
    Publication statusPublished - 2006 Aug 8

    Fingerprint

    Cyanobacteria
    Phosphorylation
    Phycobilisomes
    Genome
    Synechococcus
    Circadian Clocks
    DNA-Binding Proteins
    Phosphotransferases
    Adenosine Triphosphate
    Genes
    Proteins
    In Vitro Techniques

    Keywords

    • Biological clock
    • Phosphorelay
    • Response regulator
    • Synechococcus

    ASJC Scopus subject areas

    • Genetics
    • General

    Cite this

    A KaiC-associating SasA-RpaA two-component regulatory system as a major circadian timing mediator in cyanobacteria. / Takai, Naoki; Nakajima, Masato; Oyama, Tokitaka; Kito, Ryotaku; Sugita, Chieko; Sugita, Mamoru; Kondo, Takao; Iwasaki, Hideo.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 32, 08.08.2006, p. 12109-12114.

    Research output: Contribution to journalArticle

    Takai, Naoki ; Nakajima, Masato ; Oyama, Tokitaka ; Kito, Ryotaku ; Sugita, Chieko ; Sugita, Mamoru ; Kondo, Takao ; Iwasaki, Hideo. / A KaiC-associating SasA-RpaA two-component regulatory system as a major circadian timing mediator in cyanobacteria. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 32. pp. 12109-12114.
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