A KaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria

Hideo Iwasaki, Stanly B. Williams, Yohko Kitayama, Masahiro Ishiura, Susan S. Golden, Takao Kondo

Research output: Contribution to journalArticle

198 Citations (Scopus)

Abstract

Both regulated expression of the clock genes kaiA, kaiB, and kaiC and interactions among the Kai proteins are proposed to be important for circadian function in the cyanobacterium Synechococcus sp. strain PCC 7942. We have identified the histidine kinase SasA as a KaiC-interacting protein. SasA contains a KaiB-like sensory domain, which appears sufficient for interaction with KaiC. Disruption of the sasA gene lowered kaiBC expression and dramatically reduced amplitude of the kai expression rhythms while shortening the period. Accordingly, sasA disruption attenuated circadian expression patterns of all tested genes, some of which became arrhythmic. Continuous sasA overexpression eliminated circadian rhythms, whereas temporal overexpression changed the phase of kaiBC expression rhythm. Thus, SasA is a close associate of the cyanobacterial clock that is necessary to sustain robust circadian rhythms.

Original languageEnglish
Pages (from-to)223-233
Number of pages11
JournalCell
Volume101
Issue number2
DOIs
Publication statusPublished - 2000 Apr 14
Externally publishedYes

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Cyanobacteria
Circadian Rhythm
Histidine
Phosphotransferases
Genes
Synechococcus
Clocks
Proteins
Gene Expression
Histidine Kinase

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A KaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria. / Iwasaki, Hideo; Williams, Stanly B.; Kitayama, Yohko; Ishiura, Masahiro; Golden, Susan S.; Kondo, Takao.

In: Cell, Vol. 101, No. 2, 14.04.2000, p. 223-233.

Research output: Contribution to journalArticle

Iwasaki, Hideo ; Williams, Stanly B. ; Kitayama, Yohko ; Ishiura, Masahiro ; Golden, Susan S. ; Kondo, Takao. / A KaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria. In: Cell. 2000 ; Vol. 101, No. 2. pp. 223-233.
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