A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos

Mika Toya, Masahiro Terasawa, Kayo Nagata, Yumi Iida, Asako Sugimoto

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The assembly of a functional mitotic spindle is crucial for achieving successful mitosis. Aurora A kinase is one of the key regulators of mitotic events, including mitotic entry, centrosome maturation and spindle bipolarity. Caenorhabditis elegans Aurora A (AIR-1) is responsible for the assembly of γ-tubulin-independent microtubules in early embryos; however, the mechanism by which AIR-1 contributes to microtubule assembly during mitosis has been unclear. Here we show by live-cell imaging and RNA-mediated interference (RNAi)-based modulation of gene activity that AIR-1 has a crucial role in the assembly of chromatin-stimulated microtubules that is independent of the γ-tubulin complex. Surprisingly, the kinase activity of AIR-1 is dispensable for this process. Although the kinase-inactive form of AIR-1 was detected along the microtubules as well as on centrosomes, the kinase-active form of AIR-1 was restricted to centrosomes. Thus, we propose that AIR-1 has a kinase-dependent role at centrosomes and a kinase-independent role for stabilizing spindle microtubules and that coordination of these two roles is crucial for the assembly of mitotic spindles.

Original languageEnglish
Pages (from-to)708-715
Number of pages8
JournalNature Cell Biology
Volume13
Issue number6
DOIs
Publication statusPublished - 2011 Jun
Externally publishedYes

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Spindle Apparatus
Caenorhabditis elegans
Centrosome
Microtubules
Phosphotransferases
Embryonic Structures
Tubulin
Mitosis
Aurora Kinase A
Chromatin Assembly and Disassembly
RNA Interference
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos. / Toya, Mika; Terasawa, Masahiro; Nagata, Kayo; Iida, Yumi; Sugimoto, Asako.

In: Nature Cell Biology, Vol. 13, No. 6, 06.2011, p. 708-715.

Research output: Contribution to journalArticle

Toya, Mika ; Terasawa, Masahiro ; Nagata, Kayo ; Iida, Yumi ; Sugimoto, Asako. / A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos. In: Nature Cell Biology. 2011 ; Vol. 13, No. 6. pp. 708-715.
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