A 'macro' image analysis of fura-2 fluorescence to visualize the distribution of functional glutamate receptor subtypes in hippocampal slices

Yoshihisa Kudo, Takeshi Nakamura, Etsuro Ito

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    The distribution of functional glutamate receptor subtypes was visualized as regional changes in intracellular Ca2+ concentration induced by administration of a specific agonist for each subtype. An image of the fura-2 fluorescence from a whole hippocampal slice was directly excited at 340 or 380 nm through an optical fibre and observed via a low-magnification objective lens (× 4). A heterogeneous distribution of N-methyl-d-aspartate (NMDA) and non-NMDA subtypes was demonstrated by the ratio of fluorescence intensities before and after administration of each agonist. This 'macro' image analysis can reveal distributions of multiple functional receptors in a living brain slice.

    Original languageEnglish
    Pages (from-to)412-420
    Number of pages9
    JournalNeuroscience Research
    Volume12
    Issue number3
    DOIs
    Publication statusPublished - 1991

    Fingerprint

    Fura-2
    Glutamate Receptors
    Aspartic Acid
    Fluorescence
    Optical Fibers
    Lenses
    Brain

    Keywords

    • AMPA
    • Fura-2
    • Glutamate receptor subtypes
    • Hippocampal slice
    • Image analysis
    • Ionotropic receptor
    • Kainate
    • Metabotropic receptor
    • N-Methyl-d-aspartate (NMDA)
    • t-ACPD

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    A 'macro' image analysis of fura-2 fluorescence to visualize the distribution of functional glutamate receptor subtypes in hippocampal slices. / Kudo, Yoshihisa; Nakamura, Takeshi; Ito, Etsuro.

    In: Neuroscience Research, Vol. 12, No. 3, 1991, p. 412-420.

    Research output: Contribution to journalArticle

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