A 'macro' image analysis of fura-2 fluorescence to visualize the distribution of functional glutamate receptor subtypes in hippocampal slices

Yoshihisa Kudo, Takeshi Nakamura, Etsuro Ito

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

The distribution of functional glutamate receptor subtypes was visualized as regional changes in intracellular Ca2+ concentration induced by administration of a specific agonist for each subtype. An image of the fura-2 fluorescence from a whole hippocampal slice was directly excited at 340 or 380 nm through an optical fibre and observed via a low-magnification objective lens (× 4). A heterogeneous distribution of N-methyl-d-aspartate (NMDA) and non-NMDA subtypes was demonstrated by the ratio of fluorescence intensities before and after administration of each agonist. This 'macro' image analysis can reveal distributions of multiple functional receptors in a living brain slice.

Original languageEnglish
Pages (from-to)412-420
Number of pages9
JournalNeuroscience Research
Volume12
Issue number3
DOIs
Publication statusPublished - 1991 Nov

Keywords

  • AMPA
  • Fura-2
  • Glutamate receptor subtypes
  • Hippocampal slice
  • Image analysis
  • Ionotropic receptor
  • Kainate
  • Metabotropic receptor
  • N-Methyl-d-aspartate (NMDA)
  • t-ACPD

ASJC Scopus subject areas

  • Neuroscience(all)

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