A Magnetosome-specific GTPase from the Magnetic Bacterium Magnetospirillum magneticum AMB-1

Yoshiko Okamura, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Magnetic bacteria produce intracellular vesicles that envelope single domain magnetite crystals. Although many proteins are present in this intracellular vesicle membrane, five are specific to this membrane. A 16-kDa protein, designated Mms16, is the most abundant of the magnetosome-specific proteins, and to establish its function we cloned and sequenced its gene from Magnetospirillum magneticum AMB-1. This was achieved by determination of the N-terminal amino acid sequence of the protein following two dimensional polyacrylamide gel electrophoresis, and sequencing of the gene was performed by gene walking using anchored polymerase chain reaction. Mms16 contains a putative ATP/GTP binding motif (P-loop). Recombinant Mms16 with a hemagglutinin tag, was expressed in Escherichia coli and purified. Recombinant Mms16 protein could bind GTP and showed GTPase activity. GTP was the preferred substrate for Mms16-catalyzed nucleotide triphosphate hydrolysis. These results suggest that a novel protein specifically localized on the magnetic particle membrane, Mms16, is a GTPase. Mms16 protein showed similar characteristics to small GTPases involved in the formation of intracellular vesicles. Furthermore, addition of the GTPase inhibitor AIF4 - also inhibited magnetic particle synthesis, suggesting that GTPase is required for magnetic particles synthesis.

Original languageEnglish
Pages (from-to)48183-48188
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number51
Publication statusPublished - 2001 Dec 21
Externally publishedYes

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Magnetospirillum
Magnetosomes
GTP Phosphohydrolases
Bacteria
Guanosine Triphosphate
Proteins
Genes
Membranes
Ferrosoferric Oxide
Recombinant proteins
Intracellular Membranes
Monomeric GTP-Binding Proteins
Polymerase chain reaction
Electrophoresis, Gel, Two-Dimensional
Hemagglutinins
Electrophoresis
Recombinant Proteins
Escherichia coli
Walking
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry

Cite this

A Magnetosome-specific GTPase from the Magnetic Bacterium Magnetospirillum magneticum AMB-1. / Okamura, Yoshiko; Takeyama, Haruko; Matsunaga, Tadashi.

In: Journal of Biological Chemistry, Vol. 276, No. 51, 21.12.2001, p. 48183-48188.

Research output: Contribution to journalArticle

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