A mathematical model for the kai-protein-based chemical oscillator and clock gene expression rhythms in cyanobacteria

Fumihiko Miyoshi, Yoichi Nakayama, Kazunari Kaizu, Hideo Iwasaki, Masaru Tomita

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    In the cyanobacterium, Synechococcus elongatus, most promoters are regulated by a circadian clock under continuous light (LL) conditions. Nevertheless, the basic circadian oscillation is primarily generated by alternating KaiC phosphorylation/dephosphorylation reactions at the posttranslational level. Indeed, the KaiC phosphorylation cycle was recently reconstituted in vitro by incubating KaiA, KaiB, and KaiC proteins with ATP. However, the molecular dynamics of this chemical oscillation and the mechanism that drives the circadian transcription/translation rhythms remain unknown. In this report, the KaiC phosphorylation cycle and the gene regulatory network in the cyanobacterial circadian system have been modeled. The model reproduces the robust KaiC phosphorylation cycle in the absence of de novo gene expression as is observed in vitro, as well as its coupling to transcriptional/translational feedback in LL conditions in vivo. Moreover, the model is consistent with most previous experiments, including various combinations of genetic knockout or overexpression of kai genes. It also predicts that multiple KaiC phosphorylation states and dynamic Kai protein interactions may be required for the cyanobacterial circadian system.

    Original languageEnglish
    Pages (from-to)69-80
    Number of pages12
    JournalJournal of Biological Rhythms
    Volume22
    Issue number1
    DOIs
    Publication statusPublished - 2007 Feb

    Fingerprint

    Cyanobacteria
    phosphorylation
    Theoretical Models
    mathematical models
    Phosphorylation
    Gene Expression
    gene expression
    Proteins
    proteins
    oscillation
    Synechococcus elongatus
    Synechococcus
    Circadian Clocks
    gene overexpression
    dephosphorylation
    molecular dynamics
    Gene Regulatory Networks
    Molecular Dynamics Simulation
    circadian rhythm
    translation (genetics)

    Keywords

    • Bio-simulation
    • Circadian rhythm
    • Cyanobacteria
    • KaiC phosphorylation cycle
    • Mathematical model

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences (miscellaneous)
    • Physiology
    • Physiology (medical)

    Cite this

    A mathematical model for the kai-protein-based chemical oscillator and clock gene expression rhythms in cyanobacteria. / Miyoshi, Fumihiko; Nakayama, Yoichi; Kaizu, Kazunari; Iwasaki, Hideo; Tomita, Masaru.

    In: Journal of Biological Rhythms, Vol. 22, No. 1, 02.2007, p. 69-80.

    Research output: Contribution to journalArticle

    Miyoshi, Fumihiko ; Nakayama, Yoichi ; Kaizu, Kazunari ; Iwasaki, Hideo ; Tomita, Masaru. / A mathematical model for the kai-protein-based chemical oscillator and clock gene expression rhythms in cyanobacteria. In: Journal of Biological Rhythms. 2007 ; Vol. 22, No. 1. pp. 69-80.
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