A method for evaluating nucleosome stability with a protein-binding fluorescent dye

Hiroyuki Taguchi, Naoki Horikoshi, Yasuhiro Arimura, Hitoshi Kurumizaka

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Nucleosomes are extremely stable histone. -DNA complexes that form the building blocks of chromatin, which accommodates genomic DNA within the nucleus. The dynamic properties of chromatin play essential roles in regulating genomic DNA functions, such as DNA replication, recombination, repair, and transcription. Histones are the protein components of nucleosomes, and their diverse modifications and variants increase the versatility of nucleosome structures and their dynamics in chromatin. Therefore, a technique to evaluate the physical properties of nucleosomes would facilitate functional studies of the various nucleosomes. In this report, we describe a convenient assay for evaluating the thermal stability of nucleosomes in vitro.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalMethods
Volume70
Issue number2-3
DOIs
Publication statusPublished - 2014

Keywords

  • Chromatin
  • Histone octamer
  • Histone variant
  • Nucleosome
  • SYPRO Orange
  • Tetrasome

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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