A microfluidic mammalian cell sorter with thermal gelation polymer solution

Y. Shirasaki, M. Goto, H. Sugino, T. Arakawa, Donghyun Yoon, Jun Mizuno, Shuichi Shoji, T. Funatsu, O. Ohara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We report a development of an on-chip sorting system for fluorescently labeled mammalian cells based on a reversible sol-gel transition of thermoreversible gelation polymer controlled by local heating with infrared laser. The new system allowed to utilize the energy from the infrared laser irradiation efficiently to achieve an extensive gelation of the polymer in the channel. This enabled the new system to sort mammalian cells with 97% viability in the large channel with 50-100 μm in depth and width. Moreover, we succeeded in sorting with 100% recovery and over 99% purity by introducing a 3D flow focusing.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1571-1573
Number of pages3
Volume3
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen
Duration: 2010 Oct 32010 Oct 7

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CityGroningen
Period10/10/310/10/7

Fingerprint

Infrared lasers
Gelation
Polymer solutions
Sorting
Microfluidics
Cells
Polymers
Laser beam effects
Sol-gels
Heating
Recovery
Hot Temperature

Keywords

  • 3D focusing
  • On-chip sorting
  • Thermoreversible gelation polymer

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Shirasaki, Y., Goto, M., Sugino, H., Arakawa, T., Yoon, D., Mizuno, J., ... Ohara, O. (2010). A microfluidic mammalian cell sorter with thermal gelation polymer solution. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 3, pp. 1571-1573)

A microfluidic mammalian cell sorter with thermal gelation polymer solution. / Shirasaki, Y.; Goto, M.; Sugino, H.; Arakawa, T.; Yoon, Donghyun; Mizuno, Jun; Shoji, Shuichi; Funatsu, T.; Ohara, O.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. p. 1571-1573.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shirasaki, Y, Goto, M, Sugino, H, Arakawa, T, Yoon, D, Mizuno, J, Shoji, S, Funatsu, T & Ohara, O 2010, A microfluidic mammalian cell sorter with thermal gelation polymer solution. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 3, pp. 1571-1573, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, 10/10/3.
Shirasaki Y, Goto M, Sugino H, Arakawa T, Yoon D, Mizuno J et al. A microfluidic mammalian cell sorter with thermal gelation polymer solution. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3. 2010. p. 1571-1573
Shirasaki, Y. ; Goto, M. ; Sugino, H. ; Arakawa, T. ; Yoon, Donghyun ; Mizuno, Jun ; Shoji, Shuichi ; Funatsu, T. ; Ohara, O. / A microfluidic mammalian cell sorter with thermal gelation polymer solution. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. pp. 1571-1573
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AU - Shoji, Shuichi

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