A micromachined liquid chromatography chip with a pillar array mixer for post-column derivatization in the analysis of neurotransmitters

Muneki Isokawa, Kanki Nakanishi, Takahiro Kanamori, Huiqi Zhuang, Hayate Yamazaki, Takuo Sugaya, Dong Hyun Yoon, Tetsushi Sekiguchi, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This work describes an on-chip mixer for post-column derivatization after liquid chromatography (LC) separation. The mixer was developed as a microchannel filled with a diamond-shaped pillar array (pillar array mixer, PA-mixer), which simultaneously enhanced the derivatization reaction and prevented the diffusion of the separated analytes. By utilizing the LC chip with a PA-mixer, neurotransmitters that are otherwise undetectable without derivatization could be separated, derivatized with a fluorogenic reagent, and detected within 15 s. In this manner, an automated bioanalytical method for point-of-care diagnosis was made possible by using the LC chip.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1318-1319
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

Keywords

  • Catecholamine
  • Diagnosis
  • Fluorogenic derivatization
  • Separation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint Dive into the research topics of 'A micromachined liquid chromatography chip with a pillar array mixer for post-column derivatization in the analysis of neurotransmitters'. Together they form a unique fingerprint.

  • Cite this

    Isokawa, M., Nakanishi, K., Kanamori, T., Zhuang, H., Yamazaki, H., Sugaya, T., Yoon, D. H., Sekiguchi, T., Funatsu, T., Shoji, S., & Tsunoda, M. (2020). A micromachined liquid chromatography chip with a pillar array mixer for post-column derivatization in the analysis of neurotransmitters. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 1318-1319). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.