A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum

Satoshi Arai, Sung Chan Lee, Duanting Zhai, Madoka Suzuki, Young Tae Chang

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The dynamics of cellular heat production and propagation remains elusive at a subcellular level. Here we report the first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum (ER thermo yellow), with the highest sensitivity reported so far (3.9%/°C). Unlike nanoparticle thermometers, ER thermo yellow stains the target organelle evenly without the commonly encountered problem of aggregation, and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types. We further confirm the ability of ER thermo yellow to monitor heat production by intracellular Ca2+ changes in HeLa cells. Our thermometer anchored at nearly-zero distance from the ER, i.e. the heat source, allowed the detection of the heat as it readily dissipated, and revealed the dynamics of heat production in real time at a subcellular level.

Original languageEnglish
Article number6701
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - 2014 Oct 21

Fingerprint

Thermometers
Molecular Probes
Thermogenesis
Fluorescent Dyes
Endoplasmic Reticulum
Hot Temperature
Temperature
HeLa Cells
Organelles
Nanoparticles
Coloring Agents

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum. / Arai, Satoshi; Lee, Sung Chan; Zhai, Duanting; Suzuki, Madoka; Chang, Young Tae.

In: Scientific Reports, Vol. 4, 6701, 21.10.2014.

Research output: Contribution to journalArticle

Arai, Satoshi ; Lee, Sung Chan ; Zhai, Duanting ; Suzuki, Madoka ; Chang, Young Tae. / A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum. In: Scientific Reports. 2014 ; Vol. 4.
@article{f3d30ac357f344e985b3467ccaa6d1a0,
title = "A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum",
abstract = "The dynamics of cellular heat production and propagation remains elusive at a subcellular level. Here we report the first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum (ER thermo yellow), with the highest sensitivity reported so far (3.9{\%}/°C). Unlike nanoparticle thermometers, ER thermo yellow stains the target organelle evenly without the commonly encountered problem of aggregation, and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types. We further confirm the ability of ER thermo yellow to monitor heat production by intracellular Ca2+ changes in HeLa cells. Our thermometer anchored at nearly-zero distance from the ER, i.e. the heat source, allowed the detection of the heat as it readily dissipated, and revealed the dynamics of heat production in real time at a subcellular level.",
author = "Satoshi Arai and Lee, {Sung Chan} and Duanting Zhai and Madoka Suzuki and Chang, {Young Tae}",
year = "2014",
month = "10",
day = "21",
doi = "10.1038/srep06701",
language = "English",
volume = "4",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum

AU - Arai, Satoshi

AU - Lee, Sung Chan

AU - Zhai, Duanting

AU - Suzuki, Madoka

AU - Chang, Young Tae

PY - 2014/10/21

Y1 - 2014/10/21

N2 - The dynamics of cellular heat production and propagation remains elusive at a subcellular level. Here we report the first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum (ER thermo yellow), with the highest sensitivity reported so far (3.9%/°C). Unlike nanoparticle thermometers, ER thermo yellow stains the target organelle evenly without the commonly encountered problem of aggregation, and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types. We further confirm the ability of ER thermo yellow to monitor heat production by intracellular Ca2+ changes in HeLa cells. Our thermometer anchored at nearly-zero distance from the ER, i.e. the heat source, allowed the detection of the heat as it readily dissipated, and revealed the dynamics of heat production in real time at a subcellular level.

AB - The dynamics of cellular heat production and propagation remains elusive at a subcellular level. Here we report the first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum (ER thermo yellow), with the highest sensitivity reported so far (3.9%/°C). Unlike nanoparticle thermometers, ER thermo yellow stains the target organelle evenly without the commonly encountered problem of aggregation, and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types. We further confirm the ability of ER thermo yellow to monitor heat production by intracellular Ca2+ changes in HeLa cells. Our thermometer anchored at nearly-zero distance from the ER, i.e. the heat source, allowed the detection of the heat as it readily dissipated, and revealed the dynamics of heat production in real time at a subcellular level.

UR - http://www.scopus.com/inward/record.url?scp=84923308790&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923308790&partnerID=8YFLogxK

U2 - 10.1038/srep06701

DO - 10.1038/srep06701

M3 - Article

VL - 4

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 6701

ER -