A murine Thy-1.2 reporter vector containing a SV40 origin for rapid cloning and analysis of eukaryotic promoters

Yuzo Kadokawa, Takahiro Kusakabe, Yusuke Kamachi, Ken ichi Isobe, Hisato Kondoh, Takashi Ohyama

Research output: Contribution to journalArticle

Abstract

A new vector, pATO, was constructed for rapid cloning and analysis of eukaryotic promoters. When a recombinant pATO, carrying a promoter sequence in its multiple cloning site, was introduced into COS cells, Thy-1.2 protein was produced on the cell surface, and was easily identified by an fluorescein-conjugated anti-Thy-1.2 antibody. The intensity of the fluorescence reflected the strength of the inserted promoter. Since pATO could replicate efficiently in COS cells, the recombinant plasmids recovered from a single COS cell were sufficient to transform Escherichia coli cells. This plasmid is applicable for the rapid and labor saving cloning of promoter elements.

Original languageEnglish
Pages (from-to)277-278
Number of pages2
JournalGene
Volume153
Issue number2
DOIs
Publication statusPublished - 1995 Feb 14
Externally publishedYes

Fingerprint

COS Cells
Organism Cloning
Plasmids
Fluorescein
Fluorescence
Escherichia coli
Proteins

Keywords

  • anti-Thy-1.2 antibody
  • COS cell
  • promoter trap
  • Recombinant DNA
  • shuttle vector

ASJC Scopus subject areas

  • Genetics

Cite this

A murine Thy-1.2 reporter vector containing a SV40 origin for rapid cloning and analysis of eukaryotic promoters. / Kadokawa, Yuzo; Kusakabe, Takahiro; Kamachi, Yusuke; Isobe, Ken ichi; Kondoh, Hisato; Ohyama, Takashi.

In: Gene, Vol. 153, No. 2, 14.02.1995, p. 277-278.

Research output: Contribution to journalArticle

Kadokawa, Yuzo ; Kusakabe, Takahiro ; Kamachi, Yusuke ; Isobe, Ken ichi ; Kondoh, Hisato ; Ohyama, Takashi. / A murine Thy-1.2 reporter vector containing a SV40 origin for rapid cloning and analysis of eukaryotic promoters. In: Gene. 1995 ; Vol. 153, No. 2. pp. 277-278.
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