A neuroprotective effect of adenosine A1-receptor agonists on ischemia-induced decrease in 2-deoxyglucose uptake in rat hippocampal slices

Keiko Tominaga; Shigenobu Shibata; Shigenori Watanabe

doi:10.1016/0304-3940(92)90205-L

A neuroprotective effect of adenosine A₁-receptor agonists on ischemia-induced decrease in 2-deoxyglucose uptake in rat hippocampal slices

Keiko Tominaga, Shigenobu Shibata^*, Shigenori Watanabe

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The effects of adenosine (A) receptor agonists on ischemia-induced impairment of 2-deoxyglucose (2-DG) uptake by rat hippocampal slices was evaluated. Hippocampal slices were exposed to 20-min hypoxia + hypoglycemia (ischemia) and then returned to oxygenated and glucose-containing Krebs-Ringer solution for 6 h. Ischemia reduced 2-DG uptake in the hippocampal slices. The ischemia-induced reduction in 2-DG uptake was attenuated by pretreatment with A₁ receptor agonists but not with A₂ receptor agonists. 8-Phenyltheophylline, an A₁ receptor antagonist, ex the ischemia-induced decrease. The A₁ receptor agonist-induced neuroprotective effect was blocked by co-treatment with 8-phenyltheophylline. The present study suggests that the A₁ receptor-mediated function has a protective role in ischemia-induced decreases in glucose metabolism in hippocam slices.

Original language	English
Pages (from-to)	67-70
Number of pages	4
Journal	Neuroscience Letters
Volume	145
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(92)90205-L
Publication status	Published - 1992 Sept 28
Externally published	Yes

Keywords

2-Deoxyglucose
Adenosine
Hippocampus
In vitro
Ischemia
Neuroprotection

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/0304-3940(92)90205-L

Cite this

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title = "A neuroprotective effect of adenosine A1-receptor agonists on ischemia-induced decrease in 2-deoxyglucose uptake in rat hippocampal slices",

abstract = "The effects of adenosine (A) receptor agonists on ischemia-induced impairment of 2-deoxyglucose (2-DG) uptake by rat hippocampal slices was evaluated. Hippocampal slices were exposed to 20-min hypoxia + hypoglycemia (ischemia) and then returned to oxygenated and glucose-containing Krebs-Ringer solution for 6 h. Ischemia reduced 2-DG uptake in the hippocampal slices. The ischemia-induced reduction in 2-DG uptake was attenuated by pretreatment with A1 receptor agonists but not with A2 receptor agonists. 8-Phenyltheophylline, an A1 receptor antagonist, ex the ischemia-induced decrease. The A1 receptor agonist-induced neuroprotective effect was blocked by co-treatment with 8-phenyltheophylline. The present study suggests that the A1 receptor-mediated function has a protective role in ischemia-induced decreases in glucose metabolism in hippocam slices.",

keywords = "2-Deoxyglucose, Adenosine, Hippocampus, In vitro, Ischemia, Neuroprotection",

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AU - Tominaga, Keiko

AU - Shibata, Shigenobu

AU - Watanabe, Shigenori

PY - 1992/9/28

Y1 - 1992/9/28

N2 - The effects of adenosine (A) receptor agonists on ischemia-induced impairment of 2-deoxyglucose (2-DG) uptake by rat hippocampal slices was evaluated. Hippocampal slices were exposed to 20-min hypoxia + hypoglycemia (ischemia) and then returned to oxygenated and glucose-containing Krebs-Ringer solution for 6 h. Ischemia reduced 2-DG uptake in the hippocampal slices. The ischemia-induced reduction in 2-DG uptake was attenuated by pretreatment with A1 receptor agonists but not with A2 receptor agonists. 8-Phenyltheophylline, an A1 receptor antagonist, ex the ischemia-induced decrease. The A1 receptor agonist-induced neuroprotective effect was blocked by co-treatment with 8-phenyltheophylline. The present study suggests that the A1 receptor-mediated function has a protective role in ischemia-induced decreases in glucose metabolism in hippocam slices.

AB - The effects of adenosine (A) receptor agonists on ischemia-induced impairment of 2-deoxyglucose (2-DG) uptake by rat hippocampal slices was evaluated. Hippocampal slices were exposed to 20-min hypoxia + hypoglycemia (ischemia) and then returned to oxygenated and glucose-containing Krebs-Ringer solution for 6 h. Ischemia reduced 2-DG uptake in the hippocampal slices. The ischemia-induced reduction in 2-DG uptake was attenuated by pretreatment with A1 receptor agonists but not with A2 receptor agonists. 8-Phenyltheophylline, an A1 receptor antagonist, ex the ischemia-induced decrease. The A1 receptor agonist-induced neuroprotective effect was blocked by co-treatment with 8-phenyltheophylline. The present study suggests that the A1 receptor-mediated function has a protective role in ischemia-induced decreases in glucose metabolism in hippocam slices.

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KW - Ischemia

KW - Neuroprotection

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