A new method for identifying the amino acid attached to a particular RNA in the cell

Tsutomu Suzuki, Takuya Ueda, Kimitsuna Watanabe

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

To investigate the function of tRNAs or any other aminoacylable RNAs in vivo, it is important to be able to estimate the amounts and species of aminoacylated RNAs in living cells. We have developed a method of analyzing amino acids attached to particular tRNAs obtained from cells. After the ester bond between the amino acid and the 3'-adenosine moiety of a specific aminoacyl-tRNA is stabilized by acetylation of the amino acid with [14C]acetic anhydride, the aminoacyl-tRNA can be fished out with a solid-phase-attached DNA probe. The 14C-labeled acetylamino acid is then released from the thus purified acetyl-aminoacyl-tRNAs by alkaline treatment and detected by TLC analysis.

Original languageEnglish
Pages (from-to)195-198
Number of pages4
JournalFEBS Letters
Volume381
Issue number3
DOIs
Publication statusPublished - 1996 Mar 4
Externally publishedYes

Keywords

  • Amino acid
  • Aminoacyl-tRNA (in vivo)
  • Candida
  • Hybridization
  • N-Acetylation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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